1pjw
From Proteopedia
(New page: 200px<br /> <applet load="1pjw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pjw" /> '''Solution Structure of the Domain III of the...) |
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'''Solution Structure of the Domain III of the Japan Encephalitis Virus Envelope Protein'''<br /> | '''Solution Structure of the Domain III of the Japan Encephalitis Virus Envelope Protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The flavivirus envelope protein is the dominant antigen in eliciting | + | The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a beta-barrel type structure composed of six antiparallel beta-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses. |
==About this Structure== | ==About this Structure== | ||
| - | 1PJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Japanese_encephalitis_virus Japanese encephalitis virus]. Full crystallographic information is available from [http:// | + | 1PJW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Japanese_encephalitis_virus Japanese encephalitis virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PJW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Japanese encephalitis virus]] | [[Category: Japanese encephalitis virus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Lin, C | + | [[Category: Lin, C W.]] |
| - | [[Category: Lou, Y | + | [[Category: Lou, Y C.]] |
| - | [[Category: Tsao, Y | + | [[Category: Tsao, Y P.]] |
| - | [[Category: Wu, C | + | [[Category: Wu, C W.]] |
| - | [[Category: Wu, K | + | [[Category: Wu, K P.]] |
[[Category: e protein]] | [[Category: e protein]] | ||
[[Category: epitope mapping]] | [[Category: epitope mapping]] | ||
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[[Category: structure]] | [[Category: structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:29:34 2008'' |
Revision as of 12:29, 21 February 2008
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Solution Structure of the Domain III of the Japan Encephalitis Virus Envelope Protein
Overview
The flavivirus envelope protein is the dominant antigen in eliciting neutralizing antibodies and plays an important role in inducing immunologic responses in the infected host. We have determined the solution structure of the major antigenic domain (domain III) of the Japanese encephalitis virus (JEV) envelope protein. The JEV domain III forms a beta-barrel type structure composed of six antiparallel beta-strands resembling the immunoglobulin constant domain. We have also identified epitopes of the JEV domain III to its neutralizing antibody by chemical shift perturbation measurements. Site-directed mutagenesis experiments are performed to confirm the NMR results. Our study provides a structural basis for understanding the mechanism of immunologic protection and for rational design of vaccines effective against flaviviruses.
About this Structure
1PJW is a Single protein structure of sequence from Japanese encephalitis virus. Full crystallographic information is available from OCA.
Reference
Structural basis of a flavivirus recognized by its neutralizing antibody: solution structure of the domain III of the Japanese encephalitis virus envelope protein., Wu KP, Wu CW, Tsao YP, Kuo TW, Lou YC, Lin CW, Wu SC, Cheng JW, J Biol Chem. 2003 Nov 14;278(46):46007-13. Epub 2003 Sep 2. PMID:12952958
Page seeded by OCA on Thu Feb 21 14:29:34 2008
Categories: Japanese encephalitis virus | Single protein | Lin, C W. | Lou, Y C. | Tsao, Y P. | Wu, C W. | Wu, K P. | E protein | Epitope mapping | Flavivirus | Jev | Nmr | Structure
