1pmb
From Proteopedia
(New page: 200px<br /><applet load="1pmb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pmb, resolution 2.5Å" /> '''THE DETERMINATION OF ...) |
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| - | [[Image:1pmb.gif|left|200px]]<br /><applet load="1pmb" size=" | + | [[Image:1pmb.gif|left|200px]]<br /><applet load="1pmb" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pmb, resolution 2.5Å" /> | caption="1pmb, resolution 2.5Å" /> | ||
'''THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT'''<br /> | '''THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | As part of a protein engineering study, the X-ray crystal structure of | + | As part of a protein engineering study, the X-ray crystal structure of recombinant pig myoglobin, prepared and crystallized from E. coli, has been determined. Diffraction data were collected to 2.5 A spacing using a synchrotron X-ray source. The structure was solved using the molecular-replacement method and refined using least-squares minimization procedures to a crystallographic R factor of 18.5% using 14,481 reflections between 10 and 2.5 A. A preliminary comparison of the structure of pig myoglobin with other myoglobin structures is presented. |
==About this Structure== | ==About this Structure== | ||
| - | 1PMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with HEM as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1PMB is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa] with <scene name='pdbligand=HEM:'>HEM</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PMB OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Sus scrofa]] | [[Category: Sus scrofa]] | ||
| - | [[Category: Dodson, E | + | [[Category: Dodson, E J.]] |
| - | [[Category: Dodson, G | + | [[Category: Dodson, G G.]] |
| - | [[Category: Hubbard, R | + | [[Category: Hubbard, R E.]] |
| - | [[Category: Oldfield, T | + | [[Category: Oldfield, T J.]] |
| - | [[Category: Smerdon, S | + | [[Category: Smerdon, S J.]] |
| - | [[Category: Wilkinson, A | + | [[Category: Wilkinson, A J.]] |
[[Category: HEM]] | [[Category: HEM]] | ||
[[Category: oxygen storage]] | [[Category: oxygen storage]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:15 2008'' |
Revision as of 12:30, 21 February 2008
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THE DETERMINATION OF THE CRYSTAL STRUCTURE OF RECOMBINANT PIG MYOGLOBIN BY MOLECULAR REPLACEMENT AND ITS REFINEMENT
Overview
As part of a protein engineering study, the X-ray crystal structure of recombinant pig myoglobin, prepared and crystallized from E. coli, has been determined. Diffraction data were collected to 2.5 A spacing using a synchrotron X-ray source. The structure was solved using the molecular-replacement method and refined using least-squares minimization procedures to a crystallographic R factor of 18.5% using 14,481 reflections between 10 and 2.5 A. A preliminary comparison of the structure of pig myoglobin with other myoglobin structures is presented.
About this Structure
1PMB is a Single protein structure of sequence from Sus scrofa with as ligand. Full crystallographic information is available from OCA.
Reference
Determination of the crystal structure of recombinant pig myoglobin by molecular replacement and its refinement., Smerdon SJ, Oldfield TJ, Dodson EJ, Dodson GG, Hubbard RE, Wilkinson AJ, Acta Crystallogr B. 1990 Jun 1;46 ( Pt 3):370-7. PMID:2383370
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