1pns
From Proteopedia
(New page: 200px<br /><applet load="1pns" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pns, resolution 8.7Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1pns.jpg|left|200px]]<br /><applet load="1pns" size=" | + | [[Image:1pns.jpg|left|200px]]<br /><applet load="1pns" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pns, resolution 8.7Å" /> | caption="1pns, resolution 8.7Å" /> | ||
'''Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli, 30S Subunit of 70S Ribosome. THIS FILE, 1PNS, CONTAINS THE 30S SUBUNIT, TWO TRNAS, AND ONE MRNA MOLECULE. THE 50S RIBOSOMAL SUBUNIT IS IN FILE 1PNU.'''<br /> | '''Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli, 30S Subunit of 70S Ribosome. THIS FILE, 1PNS, CONTAINS THE 30S SUBUNIT, TWO TRNAS, AND ONE MRNA MOLECULE. THE 50S RIBOSOMAL SUBUNIT IS IN FILE 1PNU.'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Protein biosynthesis on the ribosome requires accurate reading of the | + | Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis. |
==About this Structure== | ==About this Structure== | ||
| - | 1PNS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1PNS is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PNS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Cate, J | + | [[Category: Cate, J H.D.]] |
| - | [[Category: Ridgeway, W | + | [[Category: Ridgeway, W K.]] |
[[Category: Santoso, S.]] | [[Category: Santoso, S.]] | ||
[[Category: Seymaner, V.]] | [[Category: Seymaner, V.]] | ||
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[[Category: rna-rna complex]] | [[Category: rna-rna complex]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:40 2008'' |
Revision as of 12:30, 21 February 2008
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Crystal Structure of a Streptomycin Dependent Ribosome from E. Coli, 30S Subunit of 70S Ribosome. THIS FILE, 1PNS, CONTAINS THE 30S SUBUNIT, TWO TRNAS, AND ONE MRNA MOLECULE. THE 50S RIBOSOMAL SUBUNIT IS IN FILE 1PNU.
Overview
Protein biosynthesis on the ribosome requires accurate reading of the genetic code in mRNA. Two conformational rearrangements in the small ribosomal subunit, a closing of the head and body around the incoming tRNA and an RNA helical switch near the mRNA decoding site, have been proposed to select for complementary base-pairing between mRNA codons and tRNA anticodons. We determined x-ray crystal structures of the WT and a hyper-accurate variant of the Escherichia coli ribosome at resolutions of 10 and 9 A, respectively, revealing that formation of the intact 70S ribosome from its two subunits closes the conformation of the head of the small subunit independent of mRNA decoding. Moreover, no change in the conformation of the switch helix is observed in two steps of tRNA discrimination. These 70S ribosome structures indicate that mRNA decoding is coupled primarily to movement of the small subunit body, consistent with previous proposals, whereas closing of the head and the helical switch may function in other steps of protein synthesis.
About this Structure
1PNS is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
X-ray crystal structures of the WT and a hyper-accurate ribosome from Escherichia coli., Vila-Sanjurjo A, Ridgeway WK, Seymaner V, Zhang W, Santoso S, Yu K, Cate JH, Proc Natl Acad Sci U S A. 2003 Jul 22;100(15):8682-7. Epub 2003 Jul 9. PMID:12853578
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