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1pop
From Proteopedia
(New page: 200px<br /><applet load="1pop" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pop, resolution 2.1Å" /> '''X-RAY CRYSTALLOGRAPHI...) |
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| - | [[Image:1pop.gif|left|200px]]<br /><applet load="1pop" size=" | + | [[Image:1pop.gif|left|200px]]<br /><applet load="1pop" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pop, resolution 2.1Å" /> | caption="1pop, resolution 2.1Å" /> | ||
'''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX'''<br /> | '''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of the papain-leupeptin complex has been | + | The three-dimensional structure of the papain-leupeptin complex has been determined by X-ray crystallography to a resolution of 2.1 A (overall R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts the S subsites of the papain active site and not the S' subsites; (ii) the 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25 sulphur atom of papain and is tetrahedrally coordinated; (iii) the 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes hydrogen bond contacts with Gln-19 and Cys-25. |
==About this Structure== | ==About this Structure== | ||
| - | 1POP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with ACE and MOH as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http:// | + | 1POP is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=ACE:'>ACE</scene> and <scene name='pdbligand=MOH:'>MOH</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Papain Papain], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.2 3.4.22.2] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1POP OCA]. |
==Reference== | ==Reference== | ||
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[[Category: hydrolase(thiol protease)]] | [[Category: hydrolase(thiol protease)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:30:57 2008'' |
Revision as of 12:30, 21 February 2008
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X-RAY CRYSTALLOGRAPHIC STRUCTURE OF A PAPAIN-LEUPEPTIN COMPLEX
Overview
The three-dimensional structure of the papain-leupeptin complex has been determined by X-ray crystallography to a resolution of 2.1 A (overall R-factor = 19.8%). The structure indicates that: (i) leupeptin contacts the S subsites of the papain active site and not the S' subsites; (ii) the 'carbonyl' carbon atom of the inhibitor is covalently bound by the Cys-25 sulphur atom of papain and is tetrahedrally coordinated; (iii) the 'carbonyl' oxygen atom of the inhibitor faces the oxyanion hole and makes hydrogen bond contacts with Gln-19 and Cys-25.
About this Structure
1POP is a Single protein structure of sequence from [1] with and as ligands. Active as Papain, with EC number 3.4.22.2 Full crystallographic information is available from OCA.
Reference
X-ray crystallographic structure of a papain-leupeptin complex., Schroder E, Phillips C, Garman E, Harlos K, Crawford C, FEBS Lett. 1993 Jan 2;315(1):38-42. PMID:8416808
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