Clegg TBP sandbox
From Proteopedia
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The TATA box-binding protein (TBP) is required by all three eukaryotic RNA polymerases for correct initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs. The cocrystal structure of the C-terminal/core region of human TBP complexed with the TATA element of the adenovirus major late promoter has been determined at 1.9 angstroms resolution. Structural and functional analyses of the protein-DNA complex are presented, with a detailed comparison to our 1.9-angstroms resolution structure of Arabidopsis thaliana TBP2 bound to the same TATA box. | The TATA box-binding protein (TBP) is required by all three eukaryotic RNA polymerases for correct initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs. The cocrystal structure of the C-terminal/core region of human TBP complexed with the TATA element of the adenovirus major late promoter has been determined at 1.9 angstroms resolution. Structural and functional analyses of the protein-DNA complex are presented, with a detailed comparison to our 1.9-angstroms resolution structure of Arabidopsis thaliana TBP2 bound to the same TATA box. | ||
| - | The TBP has two imperfect direct repeats that form a <scene name='Clegg_TBP_sandbox/Saddle/1'>saddle</scene> shaped structure forming a concave like DNA binding surface allowing for an induced-fit mechanism. The imperfect direct repeats differ by two residues. The first is a deletion of a resiude at the end of helix H1. The second is a proline in helix H2' that enables a <scene name='Clegg_TBP_sandbox/Proline/1'>bend</scene> that does not occur on H2. | + | The TBP has two imperfect direct repeats that form a <scene name='Clegg_TBP_sandbox/Saddle/1'>saddle</scene> shaped structure forming a concave like DNA binding surface allowing for an induced-fit mechanism. The imperfect direct repeats differ by two residues. The first is a deletion of a resiude at the end of helix H1. The second is a proline in helix H2' that enables a <scene name='Clegg_TBP_sandbox/Proline/1'>bend</scene> that does not occur on H2. When the TBP binds to the TATA box a conformation change takes place and the DNA strand is <scene name='Clegg_TBP_sandbox/Bend/1'>bent</scene> 80 degrees. This conformational change promotes the recruitment of other general transcription factors. |
==About this Structure== | ==About this Structure== | ||
Revision as of 05:18, 7 November 2012
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| 1cdw, resolution 1.90Å () | |||||||||
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| Resources: | FirstGlance, OCA, RCSB, PDBsum | ||||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||||
Contents |
HUMAN TBP CORE DOMAIN COMPLEXED WITH DNA
The TATA box-binding protein (TBP) is required by all three eukaryotic RNA polymerases for correct initiation of transcription of ribosomal, messenger, small nuclear, and transfer RNAs. The cocrystal structure of the C-terminal/core region of human TBP complexed with the TATA element of the adenovirus major late promoter has been determined at 1.9 angstroms resolution. Structural and functional analyses of the protein-DNA complex are presented, with a detailed comparison to our 1.9-angstroms resolution structure of Arabidopsis thaliana TBP2 bound to the same TATA box.
The TBP has two imperfect direct repeats that form a shaped structure forming a concave like DNA binding surface allowing for an induced-fit mechanism. The imperfect direct repeats differ by two residues. The first is a deletion of a resiude at the end of helix H1. The second is a proline in helix H2' that enables a that does not occur on H2. When the TBP binds to the TATA box a conformation change takes place and the DNA strand is 80 degrees. This conformational change promotes the recruitment of other general transcription factors.
About this Structure
1cdw is a 3 chain structure with sequence from Homo sapiens. The July 2005 RCSB PDB Molecule of the Month feature on TATA-Binding Protein by David S. Goodsell is 10.2210/rcsb_pdb/mom_2005_7. Full crystallographic information is available from OCA.
See Also
Reference
- Nikolov DB, Chen H, Halay ED, Hoffman A, Roeder RG, Burley SK. Crystal structure of a human TATA box-binding protein/TATA element complex. Proc Natl Acad Sci U S A. 1996 May 14;93(10):4862-7. PMID:8643494
- Cokol M, Nair R, Rost B. Finding nuclear localization signals. EMBO Rep. 2000 Nov;1(5):411-5. PMID:11258480 doi:10.1093/embo-reports/kvd092
- Hicks JM, Hsu VL. The extended left-handed helix: a simple nucleic acid-binding motif. Proteins. 2004 May 1;55(2):330-8. PMID:15048824 doi:10.1002/prot.10630
