1pp4
From Proteopedia
(New page: 200px<br /><applet load="1pp4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pp4, resolution 2.5Å" /> '''The crystal structure...) |
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| - | [[Image:1pp4.gif|left|200px]]<br /><applet load="1pp4" size=" | + | [[Image:1pp4.gif|left|200px]]<br /><applet load="1pp4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pp4, resolution 2.5Å" /> | caption="1pp4, resolution 2.5Å" /> | ||
'''The crystal structure of rhamnogalacturonan acetylesterase in space group P3121'''<br /> | '''The crystal structure of rhamnogalacturonan acetylesterase in space group P3121'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The glycoprotein rhamnogalacturonan acetylesterase from Aspergillus | + | The glycoprotein rhamnogalacturonan acetylesterase from Aspergillus aculeatus has been crystallized in two crystal forms, an orthorhombic and a trigonal crystal form. In the orthorhombic crystal form, the covalently bound carbohydrate at one of the two N-glycosylation sites is involved in crystal contacts. The orthorhombic crystal form was obtained at pH 5.0 and the trigonal crystal form at pH 4.5. In one case, the two crystal forms were found in the same drop at pH 4.7. The differences in crystal packing in the two crystal forms can be explained by the pH-dependent variation in the protonation state of the glutamic acid residues on the protein surface. |
==About this Structure== | ==About this Structure== | ||
| - | 1PP4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_aculeatus Aspergillus aculeatus] with NAG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1PP4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_aculeatus Aspergillus aculeatus] with <scene name='pdbligand=NAG:'>NAG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PP4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: gds(l) hydrolase]] | [[Category: gds(l) hydrolase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:02 2008'' |
Revision as of 12:31, 21 February 2008
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The crystal structure of rhamnogalacturonan acetylesterase in space group P3121
Overview
The glycoprotein rhamnogalacturonan acetylesterase from Aspergillus aculeatus has been crystallized in two crystal forms, an orthorhombic and a trigonal crystal form. In the orthorhombic crystal form, the covalently bound carbohydrate at one of the two N-glycosylation sites is involved in crystal contacts. The orthorhombic crystal form was obtained at pH 5.0 and the trigonal crystal form at pH 4.5. In one case, the two crystal forms were found in the same drop at pH 4.7. The differences in crystal packing in the two crystal forms can be explained by the pH-dependent variation in the protonation state of the glutamic acid residues on the protein surface.
About this Structure
1PP4 is a Single protein structure of sequence from Aspergillus aculeatus with as ligand. Full crystallographic information is available from OCA.
Reference
Crystal packing in two pH-dependent crystal forms of rhamnogalacturonan acetylesterase., Molgaard A, Larsen S, Acta Crystallogr D Biol Crystallogr. 2004 Mar;60(Pt 3):472-8. Epub 2004, Feb 25. PMID:14993671
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