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1ps4

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==Overview==
==Overview==
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DJ-1 is a protein involved in multiple physiological processes, including, cancer, Parkinson's disease, and male fertility. It is unknown how DJ-1, functions in the apparently different systems. The crystal structure of, DJ-1 at 1.6 A resolution shows that DJ-1 is a helix-strand-helix sandwich, and forms a dimer. The DJ-1 structure is similar to the members of the, intracellular protease PfpI family. However, the catalytic triad of, Cys-His-Glu is not strictly conserved in DJ-1, implying that DJ-1 has a, different catalytic mechanism if it acts as a protease or DJ-1 serves as a, regulatory protein in the physiological processes. The structure shows, that Leu166 positions in the middle of a helix and thus predicts that the, L166P mutation will bend the helix and impact the dimerization of DJ-1. As, a result, the conformational changes may diminish the DJ-1 binding with, its partner, leading to the familial Parkinson's disease caused by the, single L166P mutation.
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DJ-1 is a protein involved in multiple physiological processes, including cancer, Parkinson's disease, and male fertility. It is unknown how DJ-1 functions in the apparently different systems. The crystal structure of DJ-1 at 1.6 A resolution shows that DJ-1 is a helix-strand-helix sandwich and forms a dimer. The DJ-1 structure is similar to the members of the intracellular protease PfpI family. However, the catalytic triad of Cys-His-Glu is not strictly conserved in DJ-1, implying that DJ-1 has a different catalytic mechanism if it acts as a protease or DJ-1 serves as a regulatory protein in the physiological processes. The structure shows that Leu166 positions in the middle of a helix and thus predicts that the L166P mutation will bend the helix and impact the dimerization of DJ-1. As a result, the conformational changes may diminish the DJ-1 binding with its partner, leading to the familial Parkinson's disease caused by the single L166P mutation.
==Disease==
==Disease==
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[[Category: Homo sapiens]]
[[Category: Homo sapiens]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Chin, L.S.]]
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[[Category: Chin, L S.]]
[[Category: Huai, Q.]]
[[Category: Huai, Q.]]
[[Category: Ke, H.]]
[[Category: Ke, H.]]
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[[Category: regulatory protein]]
[[Category: regulatory protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri Feb 15 16:41:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:31:57 2008''

Revision as of 12:32, 21 February 2008


1ps4, resolution 1.6Å

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crystal structure of DJ-1

Contents

Overview

DJ-1 is a protein involved in multiple physiological processes, including cancer, Parkinson's disease, and male fertility. It is unknown how DJ-1 functions in the apparently different systems. The crystal structure of DJ-1 at 1.6 A resolution shows that DJ-1 is a helix-strand-helix sandwich and forms a dimer. The DJ-1 structure is similar to the members of the intracellular protease PfpI family. However, the catalytic triad of Cys-His-Glu is not strictly conserved in DJ-1, implying that DJ-1 has a different catalytic mechanism if it acts as a protease or DJ-1 serves as a regulatory protein in the physiological processes. The structure shows that Leu166 positions in the middle of a helix and thus predicts that the L166P mutation will bend the helix and impact the dimerization of DJ-1. As a result, the conformational changes may diminish the DJ-1 binding with its partner, leading to the familial Parkinson's disease caused by the single L166P mutation.

Disease

Known diseases associated with this structure: Amyotrophic lateral sclerosis-Parkinsonism/dementia complex 2 OMIM:[602533], Parkinson disease 7, autosomal recessive early-onset OMIM:[602533]

About this Structure

1PS4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of DJ-1/RS and implication on familial Parkinson's disease., Huai Q, Sun Y, Wang H, Chin LS, Li L, Robinson H, Ke H, FEBS Lett. 2003 Aug 14;549(1-3):171-5. PMID:12914946

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