1pzw
From Proteopedia
(New page: 200px<br /><applet load="1pzw" size="450" color="white" frame="true" align="right" spinBox="true" caption="1pzw, resolution 2.00Å" /> '''Crystal structure of...) |
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| - | [[Image:1pzw.gif|left|200px]]<br /><applet load="1pzw" size=" | + | [[Image:1pzw.gif|left|200px]]<br /><applet load="1pzw" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1pzw, resolution 2.00Å" /> | caption="1pzw, resolution 2.00Å" /> | ||
'''Crystal structure of the zinc finger associated domain of the Drosophila transcription factor Grauzone'''<br /> | '''Crystal structure of the zinc finger associated domain of the Drosophila transcription factor Grauzone'''<br /> | ||
==Overview== | ==Overview== | ||
| - | About one-third of the more than 300 C2H2 zinc finger proteins of | + | About one-third of the more than 300 C2H2 zinc finger proteins of Drosophila contain a conserved sequence motif, the zinc finger-associated domain (ZAD). Genes that encode ZAD proteins are specific for and expanded in the genomes of insects. Only three ZAD-encoding gene functions are established, and the role of ZAD is unknown. Here we present the crystal structure of the ZAD of Grauzone (ZAD(Grau)), a Drosophila transcription factor that specifically controls the maternal Cdc20-like APC subunit Cortex. ZAD forms an atypical treble-clef-like zinc-coordinating fold. Head-to-tail arrangement of two ZAD(Grau) molecules in the crystals suggests dimer formation, an observation supported by crosslinking and dynamic light scattering. The results indicate that ZAD provides a novel protein-protein interaction module that characterizes a large family of insect transcription factors. |
==About this Structure== | ==About this Structure== | ||
| - | 1PZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1PZW is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PZW OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bourenkov, G | + | [[Category: Bourenkov, G P.]] |
| - | [[Category: Chung, H | + | [[Category: Chung, H R.]] |
[[Category: Jaeckle, H.]] | [[Category: Jaeckle, H.]] | ||
[[Category: Jauch, R.]] | [[Category: Jauch, R.]] | ||
[[Category: Reidt, U.]] | [[Category: Reidt, U.]] | ||
[[Category: Urlaub, H.]] | [[Category: Urlaub, H.]] | ||
| - | [[Category: Wahl, M | + | [[Category: Wahl, M C.]] |
[[Category: ZN]] | [[Category: ZN]] | ||
[[Category: dimerization]] | [[Category: dimerization]] | ||
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[[Category: zad]] | [[Category: zad]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:20 2008'' |
Revision as of 12:34, 21 February 2008
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Crystal structure of the zinc finger associated domain of the Drosophila transcription factor Grauzone
Overview
About one-third of the more than 300 C2H2 zinc finger proteins of Drosophila contain a conserved sequence motif, the zinc finger-associated domain (ZAD). Genes that encode ZAD proteins are specific for and expanded in the genomes of insects. Only three ZAD-encoding gene functions are established, and the role of ZAD is unknown. Here we present the crystal structure of the ZAD of Grauzone (ZAD(Grau)), a Drosophila transcription factor that specifically controls the maternal Cdc20-like APC subunit Cortex. ZAD forms an atypical treble-clef-like zinc-coordinating fold. Head-to-tail arrangement of two ZAD(Grau) molecules in the crystals suggests dimer formation, an observation supported by crosslinking and dynamic light scattering. The results indicate that ZAD provides a novel protein-protein interaction module that characterizes a large family of insect transcription factors.
About this Structure
1PZW is a Single protein structure of sequence from Drosophila melanogaster with as ligand. Full crystallographic information is available from OCA.
Reference
The zinc finger-associated domain of the Drosophila transcription factor grauzone is a novel zinc-coordinating protein-protein interaction module., Jauch R, Bourenkov GP, Chung HR, Urlaub H, Reidt U, Jackle H, Wahl MC, Structure. 2003 Nov;11(11):1393-402. PMID:14604529
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