1q0s
From Proteopedia
(New page: 200px<br /><applet load="1q0s" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q0s, resolution 2.3Å" /> '''Binary Structure of T...) |
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| - | [[Image:1q0s.jpg|left|200px]]<br /><applet load="1q0s" size=" | + | [[Image:1q0s.jpg|left|200px]]<br /><applet load="1q0s" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1q0s, resolution 2.3Å" /> | caption="1q0s, resolution 2.3Å" /> | ||
'''Binary Structure of T4DAM with AdoHcy'''<br /> | '''Binary Structure of T4DAM with AdoHcy'''<br /> | ||
==Overview== | ==Overview== | ||
| - | DNA-adenine methylation at certain GATC sites plays a pivotal role in | + | DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q0S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with SAH as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] Full crystallographic information is available from [http:// | + | 1Q0S is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacteriophage_t4 Bacteriophage t4] with <scene name='pdbligand=SAH:'>SAH</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Site-specific_DNA-methyltransferase_(adenine-specific) Site-specific DNA-methyltransferase (adenine-specific)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.72 2.1.1.72] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q0S OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Dong, A.]] | [[Category: Dong, A.]] | ||
[[Category: Hattman, S.]] | [[Category: Hattman, S.]] | ||
| - | [[Category: Horton, J | + | [[Category: Horton, J R.]] |
[[Category: Kossykh, V.]] | [[Category: Kossykh, V.]] | ||
| - | [[Category: Schlagman, S | + | [[Category: Schlagman, S L.]] |
[[Category: Yang, Z.]] | [[Category: Yang, Z.]] | ||
[[Category: Zhang, X.]] | [[Category: Zhang, X.]] | ||
| - | [[Category: Zhang, X | + | [[Category: Zhang, X J.]] |
[[Category: Zhou, L.]] | [[Category: Zhou, L.]] | ||
[[Category: SAH]] | [[Category: SAH]] | ||
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[[Category: t4dam]] | [[Category: t4dam]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:36 2008'' |
Revision as of 12:34, 21 February 2008
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Binary Structure of T4DAM with AdoHcy
Overview
DNA-adenine methylation at certain GATC sites plays a pivotal role in bacterial and phage gene expression as well as bacterial virulence. We report here the crystal structures of the bacteriophage T4Dam DNA adenine methyltransferase (MTase) in a binary complex with the methyl-donor product S-adenosyl-L-homocysteine (AdoHcy) and in a ternary complex with a synthetic 12-bp DNA duplex and AdoHcy. T4Dam contains two domains: a seven-stranded catalytic domain that harbors the binding site for AdoHcy and a DNA binding domain consisting of a five-helix bundle and a beta-hairpin that is conserved in the family of GATC-related MTase orthologs. Unexpectedly, the sequence-specific T4Dam bound to DNA in a nonspecific mode that contained two Dam monomers per synthetic duplex, even though the DNA contains a single GATC site. The ternary structure provides a rare snapshot of an enzyme poised for linear diffusion along the DNA.
About this Structure
1Q0S is a Single protein structure of sequence from Bacteriophage t4 with as ligand. Active as Site-specific DNA-methyltransferase (adenine-specific), with EC number 2.1.1.72 Full crystallographic information is available from OCA.
Reference
Structure of the bacteriophage T4 DNA adenine methyltransferase., Yang Z, Horton JR, Zhou L, Zhang XJ, Dong A, Zhang X, Schlagman SL, Kossykh V, Hattman S, Cheng X, Nat Struct Biol. 2003 Oct;10(10):849-55. Epub 2003 Aug 24. PMID:12937411
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