1q1j
From Proteopedia
(New page: 200px<br /> <applet load="1q1j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q1j, resolution 2.50Å" /> '''Crystal Structure A...) |
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caption="1q1j, resolution 2.50Å" /> | caption="1q1j, resolution 2.50Å" /> | ||
'''Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide'''<br /> | '''Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide'''<br /> | ||
==Overview== | ==Overview== | ||
| - | 447-52D is a human monoclonal antibody isolated from a heterohybridoma | + | 447-52D is a human monoclonal antibody isolated from a heterohybridoma derived from an HIV-1-infected individual. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date. The crystal structure of the 447-52D Fab in complex with a 16-mer V3 peptide at 2.5 A resolution reveals that the peptide beta hairpin forms a three-stranded mixed beta sheet with complementarity determining region (CDR) H3, with most of the V3 side chains exposed to solvent. Sequence specificity is conferred through interaction of the type-II turn (residues GPGR) at the apex of the V3 hairpin with the base of CDR H3. This novel mode of peptide-antibody recognition enables the antibody to bind to many different V3 sequences where only the GPxR core epitope is absolutely required. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q1J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1Q1J is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q1J OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Gorny, M | + | [[Category: Gorny, M K.]] |
| - | [[Category: Stanfield, R | + | [[Category: Stanfield, R L.]] |
[[Category: Williams, C.]] | [[Category: Williams, C.]] | ||
| - | [[Category: Wilson, I | + | [[Category: Wilson, I A.]] |
[[Category: Zolla-Pazner, S.]] | [[Category: Zolla-Pazner, S.]] | ||
[[Category: fab-peptide complex]] | [[Category: fab-peptide complex]] | ||
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[[Category: v3 loop]] | [[Category: v3 loop]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:34:55 2008'' |
Revision as of 12:35, 21 February 2008
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Crystal Structure Analysis of anti-HIV-1 Fab 447-52D in complex with V3 peptide
Overview
447-52D is a human monoclonal antibody isolated from a heterohybridoma derived from an HIV-1-infected individual. This antibody recognizes the hypervariable gp120 V3 loop, and neutralizes both X4 and R5 primary isolates, making it one of the most effective anti-V3 antibodies characterized to date. The crystal structure of the 447-52D Fab in complex with a 16-mer V3 peptide at 2.5 A resolution reveals that the peptide beta hairpin forms a three-stranded mixed beta sheet with complementarity determining region (CDR) H3, with most of the V3 side chains exposed to solvent. Sequence specificity is conferred through interaction of the type-II turn (residues GPGR) at the apex of the V3 hairpin with the base of CDR H3. This novel mode of peptide-antibody recognition enables the antibody to bind to many different V3 sequences where only the GPxR core epitope is absolutely required.
About this Structure
1Q1J is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural rationale for the broad neutralization of HIV-1 by human monoclonal antibody 447-52D., Stanfield RL, Gorny MK, Williams C, Zolla-Pazner S, Wilson IA, Structure. 2004 Feb;12(2):193-204. PMID:14962380
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