1q2h
From Proteopedia
(New page: 200px<br /> <applet load="1q2h" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q2h, resolution 1.70Å" /> '''Phenylalanine Zippe...) |
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| - | [[Image:1q2h.gif|left|200px]]<br /> | + | [[Image:1q2h.gif|left|200px]]<br /><applet load="1q2h" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1q2h" size=" | + | |
caption="1q2h, resolution 1.70Å" /> | caption="1q2h, resolution 1.70Å" /> | ||
'''Phenylalanine Zipper Mediates APS Dimerization'''<br /> | '''Phenylalanine Zipper Mediates APS Dimerization'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The APS, SH2-B and LNK proteins are adapters that activate and modulate | + | The APS, SH2-B and LNK proteins are adapters that activate and modulate receptor tyrosine kinase and JAK/STAT signaling. We now show that a conserved N-terminal domain mediates APS homodimerization. We determined the crystal structure of the dimerization domain at a resolution of 1.7 A using bromide ion MAD phasing. Each molecule contributes two helices to a compact four-helix bundle having a bisecting-U topology. Its most conspicuous feature is a stack of interdigitated phenylalanine side chains at the domain core. These residues create a new motif we refer to as a 'phenylalanine zipper,' which is critical to dimerization. A newly developed bridging yeast tri-hybrid assay showed that APS dimerizes JAK2, insulin receptor and IGF1 receptor kinases using its SH2 and dimerization domains. Dimerization via the phenylalanine zipper domain provides a mechanism for activating and modulating tyrosine kinase activity even in the absence of extracellular ligands. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1Q2H is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q2H OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chi, Y | + | [[Category: Chi, Y I.]] |
[[Category: Dhe-Paganon, S.]] | [[Category: Dhe-Paganon, S.]] | ||
[[Category: Nishi, M.]] | [[Category: Nishi, M.]] | ||
| - | [[Category: Shoelson, S | + | [[Category: Shoelson, S E.]] |
| - | [[Category: Werner, E | + | [[Category: Werner, E D.]] |
[[Category: signal transduction]] | [[Category: signal transduction]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:35:16 2008'' |
Revision as of 12:35, 21 February 2008
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Phenylalanine Zipper Mediates APS Dimerization
Overview
The APS, SH2-B and LNK proteins are adapters that activate and modulate receptor tyrosine kinase and JAK/STAT signaling. We now show that a conserved N-terminal domain mediates APS homodimerization. We determined the crystal structure of the dimerization domain at a resolution of 1.7 A using bromide ion MAD phasing. Each molecule contributes two helices to a compact four-helix bundle having a bisecting-U topology. Its most conspicuous feature is a stack of interdigitated phenylalanine side chains at the domain core. These residues create a new motif we refer to as a 'phenylalanine zipper,' which is critical to dimerization. A newly developed bridging yeast tri-hybrid assay showed that APS dimerizes JAK2, insulin receptor and IGF1 receptor kinases using its SH2 and dimerization domains. Dimerization via the phenylalanine zipper domain provides a mechanism for activating and modulating tyrosine kinase activity even in the absence of extracellular ligands.
About this Structure
1Q2H is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
A phenylalanine zipper mediates APS dimerization., Dhe-Paganon S, Werner ED, Nishi M, Hansen L, Chi YI, Shoelson SE, Nat Struct Mol Biol. 2004 Oct;11(10):968-74. Epub 2004 Sep 19. PMID:15378031
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