This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1bs0
From Proteopedia
| Line 27: | Line 27: | ||
[[Category: plp-dependent acyl-coa synthase]] | [[Category: plp-dependent acyl-coa synthase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:56:58 2007'' |
Revision as of 12:52, 30 October 2007
|
PLP-DEPENDENT ACYL-COA SYNTHASE
Overview
8-Amino-7-oxononanoate synthase (or 8-amino-7-ketopelargonate synthase; EC, 2.3.1.47; AONS) catalyses the decarboxylative condensation of l-alanine, and pimeloyl-CoA in the first committed step of biotin biosynthesis. We, have cloned, over-expressed and purified AONS from Escherichia coli and, determined the crystal structures of the apo and PLP-bound forms of the, enzyme. The protein is a symmetrical homodimer with a tertiary structure, and active site organisation similar to, but distinct from, those of other, PLP-dependent enzymes whose three-dimensional structures are known. The, critical PLP-binding lysine of AONS is located at the end of a deep cleft, that allows access of the pantothenate arm of pimeloyl-CoA. A cluster of, positively charged residues at the entrance to this cleft ... [(full description)]
About this Structure
1BS0 is a [Single protein] structure of sequence from [Escherichia coli] with SO4 as [ligand]. Active as [8-amino-7-oxononanoate synthase], with EC number [2.3.1.47]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].
Reference
The crystal structure of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl-CoA-condensing enzyme., Alexeev D, Alexeeva M, Baxter RL, Campopiano DJ, Webster SP, Sawyer L, J Mol Biol. 1998 Nov 27;284(2):401-19. PMID:9813126
Page seeded by OCA on Tue Oct 30 14:56:58 2007
Categories: 8-amino-7-oxononanoate synthase | Escherichia coli | Single protein | Alexeev, D. | Alexeeva, M. | Baxter, R.L. | Campopiano, D.J. | Sawyer, L. | Webster, S.P. | SO4 | 8-amino-7-ketopelargonate synthase | 8-amino-7-oxonanoate synthase | Biotin biosynthesis | Plp-dependent acyl-coa synthase
