1q5f
From Proteopedia
(New page: 200px<br /><applet load="1q5f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q5f" /> '''NMR Structure of Type IVb pilin (PilS) from ...) |
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| - | [[Image:1q5f.jpg|left|200px]]<br /><applet load="1q5f" size=" | + | [[Image:1q5f.jpg|left|200px]]<br /><applet load="1q5f" size="350" color="white" frame="true" align="right" spinBox="true" |
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'''NMR Structure of Type IVb pilin (PilS) from Salmonella typhi'''<br /> | '''NMR Structure of Type IVb pilin (PilS) from Salmonella typhi'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the N-terminal-truncated Type IVb structural pilin | + | The structure of the N-terminal-truncated Type IVb structural pilin (t-PilS) from Salmonella typhi was determined by NMR. Although topologically similar to the recently determined x-ray structure of pilin from Vibrio cholerae toxin-coregulated pilus, the only Type IVb pilin with known structure, t-PilS contains many distinct structural features. The protein contains an extra pair of beta-strands in the N-terminal alphabeta loop that align with the major beta-strands to form a continuous 7-stranded antiparallel beta-sheet. The C-terminal disulfide-bonded region of t-PilS is only half the length of that of toxin-coregulated pilus pilin. A model of S. typhi pilus has been proposed and mutagenesis studies suggested that residues on both the alphabeta loop and the C-terminal disulfide-bonded region of PilS might be involved in binding specificity of the pilus. This model structure reveals an exposed surface between adjacent subunits of PilS that could be a potential binding site for the cystic fibrosis transmembrane conductance regulator. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhi Salmonella typhi]. Full crystallographic information is available from [http:// | + | 1Q5F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Salmonella_typhi Salmonella typhi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Hackett, J.]] | [[Category: Hackett, J.]] | ||
| - | [[Category: Mok, Y | + | [[Category: Mok, Y K.]] |
| - | [[Category: Tan, Y | + | [[Category: Tan, Y W.]] |
| - | [[Category: Xu, X | + | [[Category: Xu, X F.]] |
[[Category: Zhang, M.]] | [[Category: Zhang, M.]] | ||
[[Category: alpha-beta roll]] | [[Category: alpha-beta roll]] | ||
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[[Category: type ivb pilin]] | [[Category: type ivb pilin]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:01 2008'' |
Revision as of 12:36, 21 February 2008
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NMR Structure of Type IVb pilin (PilS) from Salmonella typhi
Overview
The structure of the N-terminal-truncated Type IVb structural pilin (t-PilS) from Salmonella typhi was determined by NMR. Although topologically similar to the recently determined x-ray structure of pilin from Vibrio cholerae toxin-coregulated pilus, the only Type IVb pilin with known structure, t-PilS contains many distinct structural features. The protein contains an extra pair of beta-strands in the N-terminal alphabeta loop that align with the major beta-strands to form a continuous 7-stranded antiparallel beta-sheet. The C-terminal disulfide-bonded region of t-PilS is only half the length of that of toxin-coregulated pilus pilin. A model of S. typhi pilus has been proposed and mutagenesis studies suggested that residues on both the alphabeta loop and the C-terminal disulfide-bonded region of PilS might be involved in binding specificity of the pilus. This model structure reveals an exposed surface between adjacent subunits of PilS that could be a potential binding site for the cystic fibrosis transmembrane conductance regulator.
About this Structure
1Q5F is a Single protein structure of sequence from Salmonella typhi. Full crystallographic information is available from OCA.
Reference
NMR structure of a type IVb pilin from Salmonella typhi and its assembly into pilus., Xu XF, Tan YW, Lam L, Hackett J, Zhang M, Mok YK, J Biol Chem. 2004 Jul 23;279(30):31599-605. Epub 2004 May 24. PMID:15159389
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