1q5y
From Proteopedia
(New page: 200px<br /><applet load="1q5y" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q5y, resolution 1.40Å" /> '''Nickel-Bound C-termi...) |
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| - | [[Image:1q5y.jpg|left|200px]]<br /><applet load="1q5y" size=" | + | [[Image:1q5y.jpg|left|200px]]<br /><applet load="1q5y" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1q5y, resolution 1.40Å" /> | caption="1q5y, resolution 1.40Å" /> | ||
'''Nickel-Bound C-terminal Regulatory Domain of NikR'''<br /> | '''Nickel-Bound C-terminal Regulatory Domain of NikR'''<br /> | ||
==Overview== | ==Overview== | ||
| - | NikR is a metal-responsive transcription factor that controls nickel | + | NikR is a metal-responsive transcription factor that controls nickel uptake in Escherichia coli by regulating expression of a nickel-specific ATP-binding cassette (ABC) transporter. We have determined the first two structures of NikR: the full-length apo repressor at a resolution of 2.3 A and the nickel-bound C-terminal regulatory domain at a resolution of 1.4 A. NikR is the only known metal-responsive member of the ribbon-helix-helix family of transcription factors, and its structure has a quaternary arrangement consisting of two dimeric DNA-binding domains separated by a tetrameric regulatory domain that binds nickel. The position of the C-terminal regulatory domain enforces a large spacing between the contacts that each NikR DNA-binding domain can make with the nik operator. The regulatory domain of NikR contains four nickel-binding sites at the tetramer interface, each exhibiting a novel square-planar coordination by three histidines and one cysteine side chain. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with NI and EDO as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1Q5Y is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=NI:'>NI</scene> and <scene name='pdbligand=EDO:'>EDO</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q5Y OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chivers, P | + | [[Category: Chivers, P T.]] |
| - | [[Category: Drennan, C | + | [[Category: Drennan, C L.]] |
[[Category: Guo, Y.]] | [[Category: Guo, Y.]] | ||
| - | [[Category: Sauer, R | + | [[Category: Sauer, R T.]] |
| - | [[Category: Schreiter, E | + | [[Category: Schreiter, E R.]] |
| - | [[Category: Sintchak, M | + | [[Category: Sintchak, M D.]] |
[[Category: EDO]] | [[Category: EDO]] | ||
[[Category: NI]] | [[Category: NI]] | ||
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[[Category: regulatory domain]] | [[Category: regulatory domain]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:14 2008'' |
Revision as of 12:36, 21 February 2008
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Nickel-Bound C-terminal Regulatory Domain of NikR
Overview
NikR is a metal-responsive transcription factor that controls nickel uptake in Escherichia coli by regulating expression of a nickel-specific ATP-binding cassette (ABC) transporter. We have determined the first two structures of NikR: the full-length apo repressor at a resolution of 2.3 A and the nickel-bound C-terminal regulatory domain at a resolution of 1.4 A. NikR is the only known metal-responsive member of the ribbon-helix-helix family of transcription factors, and its structure has a quaternary arrangement consisting of two dimeric DNA-binding domains separated by a tetrameric regulatory domain that binds nickel. The position of the C-terminal regulatory domain enforces a large spacing between the contacts that each NikR DNA-binding domain can make with the nik operator. The regulatory domain of NikR contains four nickel-binding sites at the tetramer interface, each exhibiting a novel square-planar coordination by three histidines and one cysteine side chain.
About this Structure
1Q5Y is a Single protein structure of sequence from Escherichia coli with and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of the nickel-responsive transcription factor NikR., Schreiter ER, Sintchak MD, Guo Y, Chivers PT, Sauer RT, Drennan CL, Nat Struct Biol. 2003 Oct;10(10):794-9. Epub 2003 Sep 14. PMID:12970756
Page seeded by OCA on Thu Feb 21 14:36:14 2008
