1q7o
From Proteopedia
(New page: 200px<br /><applet load="1q7o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q7o" /> '''Determination of f-MLF-OH Peptide Structure ...) |
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| - | [[Image:1q7o.jpg|left|200px]]<br /><applet load="1q7o" size=" | + | [[Image:1q7o.jpg|left|200px]]<br /><applet load="1q7o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1q7o" /> | caption="1q7o" /> | ||
'''Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy'''<br /> | '''Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of the chemotactic peptide | + | The three-dimensional structure of the chemotactic peptide N-formyl-l-Met-l-Leu-l-Phe-OH was determined by using solid-state NMR (SSNMR). The set of SSNMR data consisted of 16 (13)C-(15)N distances and 18 torsion angle constraints (on 10 angles), recorded from uniformly (13)C,(15)N- and (15)N-labeled samples. The peptide's structure was calculated by means of simulated annealing and a newly developed protocol that ensures that all of conformational space, consistent with the structural constraints, is searched completely. The result is a high-quality structure of a molecule that has thus far not been amenable to single-crystal diffraction studies. The extensions of the SSNMR techniques and computational methods to larger systems appear promising. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1Q7O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7O OCA]. |
==Reference== | ==Reference== | ||
De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy., Rienstra CM, Tucker-Kellogg L, Jaroniec CP, Hohwy M, Reif B, McMahon MT, Tidor B, Lozano-Perez T, Griffin RG, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10260-5. Epub 2002 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12149447 12149447] | De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy., Rienstra CM, Tucker-Kellogg L, Jaroniec CP, Hohwy M, Reif B, McMahon MT, Tidor B, Lozano-Perez T, Griffin RG, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10260-5. Epub 2002 Jul 29. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=12149447 12149447] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Griffin, R | + | [[Category: Griffin, R G.]] |
[[Category: Hohwy, M.]] | [[Category: Hohwy, M.]] | ||
| - | [[Category: Jaroniec, C | + | [[Category: Jaroniec, C P.]] |
[[Category: Lozano-Perez, T.]] | [[Category: Lozano-Perez, T.]] | ||
| - | [[Category: McMahon, M | + | [[Category: McMahon, M T.]] |
[[Category: Reif, B.]] | [[Category: Reif, B.]] | ||
| - | [[Category: Rienstra, C | + | [[Category: Rienstra, C M.]] |
[[Category: Tidor, B.]] | [[Category: Tidor, B.]] | ||
[[Category: Tucker-Kellogg, L.]] | [[Category: Tucker-Kellogg, L.]] | ||
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[[Category: mlf]] | [[Category: mlf]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:42 2008'' |
Revision as of 12:36, 21 February 2008
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Determination of f-MLF-OH Peptide Structure with solid-state magic-angle spinning NMR Spectroscopy
Overview
The three-dimensional structure of the chemotactic peptide N-formyl-l-Met-l-Leu-l-Phe-OH was determined by using solid-state NMR (SSNMR). The set of SSNMR data consisted of 16 (13)C-(15)N distances and 18 torsion angle constraints (on 10 angles), recorded from uniformly (13)C,(15)N- and (15)N-labeled samples. The peptide's structure was calculated by means of simulated annealing and a newly developed protocol that ensures that all of conformational space, consistent with the structural constraints, is searched completely. The result is a high-quality structure of a molecule that has thus far not been amenable to single-crystal diffraction studies. The extensions of the SSNMR techniques and computational methods to larger systems appear promising.
About this Structure
1Q7O is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
De novo determination of peptide structure with solid-state magic-angle spinning NMR spectroscopy., Rienstra CM, Tucker-Kellogg L, Jaroniec CP, Hohwy M, Reif B, McMahon MT, Tidor B, Lozano-Perez T, Griffin RG, Proc Natl Acad Sci U S A. 2002 Aug 6;99(16):10260-5. Epub 2002 Jul 29. PMID:12149447
Page seeded by OCA on Thu Feb 21 14:36:42 2008
