1q7f
From Proteopedia
(New page: 200px<br /><applet load="1q7f" size="450" color="white" frame="true" align="right" spinBox="true" caption="1q7f, resolution 1.95Å" /> '''Brain Tumor NHL doma...) |
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| - | [[Image:1q7f.jpg|left|200px]]<br /><applet load="1q7f" size=" | + | [[Image:1q7f.jpg|left|200px]]<br /><applet load="1q7f" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1q7f, resolution 1.95Å" /> | caption="1q7f, resolution 1.95Å" /> | ||
'''Brain Tumor NHL domain'''<br /> | '''Brain Tumor NHL domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The Brain Tumor (Brat) protein is recruited to the 3' untranslated region | + | The Brain Tumor (Brat) protein is recruited to the 3' untranslated region (UTR) of hunchback mRNA to regulate its translation. Recruitment is mediated by interactions between the Pumilio RNA-binding Puf repeats and the NHL domain of Brat, a conserved structural motif present in a large family of growth regulators. In this report, we describe the crystal structure of the Brat NHL domain and present a model of the Pumilio-Brat complex derived from in silico docking experiments and supported by mutational analysis of the protein-protein interface. A key feature of the model is recognition of the outer, convex surface of the Pumilio Puf domain by the top, electropositive face of the six-bladed Brat beta-propeller. In particular, an extended loop in Puf repeat 8 fits in the entrance to the central channel of the Brat beta-propeller. Together, these interactions are likely to be prototypic of the recruitment strategies of other NHL-containing proteins in development. |
==About this Structure== | ==About this Structure== | ||
| - | 1Q7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http:// | + | 1Q7F is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Q7F OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Drosophila melanogaster]] | [[Category: Drosophila melanogaster]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Aggarwal, A | + | [[Category: Aggarwal, A K.]] |
| - | [[Category: Edwards, T | + | [[Category: Edwards, T A.]] |
| - | [[Category: Wharton, R | + | [[Category: Wharton, R P.]] |
| - | [[Category: Wilkinson, B | + | [[Category: Wilkinson, B D.]] |
[[Category: beta-propeller]] | [[Category: beta-propeller]] | ||
[[Category: brat]] | [[Category: brat]] | ||
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[[Category: nhl repeat]] | [[Category: nhl repeat]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:36:44 2008'' |
Revision as of 12:36, 21 February 2008
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Brain Tumor NHL domain
Overview
The Brain Tumor (Brat) protein is recruited to the 3' untranslated region (UTR) of hunchback mRNA to regulate its translation. Recruitment is mediated by interactions between the Pumilio RNA-binding Puf repeats and the NHL domain of Brat, a conserved structural motif present in a large family of growth regulators. In this report, we describe the crystal structure of the Brat NHL domain and present a model of the Pumilio-Brat complex derived from in silico docking experiments and supported by mutational analysis of the protein-protein interface. A key feature of the model is recognition of the outer, convex surface of the Pumilio Puf domain by the top, electropositive face of the six-bladed Brat beta-propeller. In particular, an extended loop in Puf repeat 8 fits in the entrance to the central channel of the Brat beta-propeller. Together, these interactions are likely to be prototypic of the recruitment strategies of other NHL-containing proteins in development.
About this Structure
1Q7F is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Model of the brain tumor-Pumilio translation repressor complex., Edwards TA, Wilkinson BD, Wharton RP, Aggarwal AK, Genes Dev. 2003 Oct 15;17(20):2508-13. PMID:14561773
Page seeded by OCA on Thu Feb 21 14:36:44 2008
