1qaz
From Proteopedia
(New page: 200px<br /><applet load="1qaz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qaz, resolution 1.78Å" /> '''CRYSTAL STRUCTURE OF...) |
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| - | [[Image:1qaz.gif|left|200px]]<br /><applet load="1qaz" size=" | + | [[Image:1qaz.gif|left|200px]]<br /><applet load="1qaz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qaz, resolution 1.78Å" /> | caption="1qaz, resolution 1.78Å" /> | ||
'''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION'''<br /> | '''CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The three-dimensional structure of alginate lyase A1-III (ALYIII) from a | + | The three-dimensional structure of alginate lyase A1-III (ALYIII) from a Sphingomonas species A1 was determined by X-ray crystallography. The enzyme was crystallized by the hanging-drop vapour-diffusion method in the presence of 49% ammonium sulfate at 20 degrees C. The crystals are monoclinic and belong to the space group C2 with unit cell dimensions of a=49.18 A, b=93.08 A, c=82.10 A and beta=104.12 degrees. There was one molecule of alginate lyase in the asymmetric unit of the crystal. The diffraction data up to 1. 71 A were collected with Rsymof 5.0%. The crystal structure of ALYIII was solved by the multiple isomorphous replacement method and refined at 1.78 A resolution using X-PLOR with a final R -factor of 18.0% for 10.0 to 1.78 A resolution data. The refined model of ALYIII contained 351 amino acid residues, 299 water molecules and two sulfate ions. The three-dimensional structure of ALYIII was abundant in helices and had a deep tunnel-like cleft in a novel (alpha6/alpha5)-barrel structure, which was similar to the (alpha6/alpha6)-barrel found in glucoamylase and cellulase. This structure presented the possibility that alginate molecules might penetrate into the cleft to interact with the catalytic site of ALYIII. |
==About this Structure== | ==About this Structure== | ||
| - | 1QAZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urea_carboxylase Urea carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.6 6.3.4.6] Full crystallographic information is available from [http:// | + | 1QAZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sphingomonas_sp. Sphingomonas sp.] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Urea_carboxylase Urea carboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.4.6 6.3.4.6] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QAZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Sphingomonas sp.]] | [[Category: Sphingomonas sp.]] | ||
[[Category: Urea carboxylase]] | [[Category: Urea carboxylase]] | ||
| - | [[Category: Yoon, H | + | [[Category: Yoon, H J.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: alginate lyase]] | [[Category: alginate lyase]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:48 2008'' |
Revision as of 12:37, 21 February 2008
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CRYSTAL STRUCTURE OF ALGINATE LYASE A1-III FROM SPHINGOMONAS SPECIES A1 AT 1.78A RESOLUTION
Overview
The three-dimensional structure of alginate lyase A1-III (ALYIII) from a Sphingomonas species A1 was determined by X-ray crystallography. The enzyme was crystallized by the hanging-drop vapour-diffusion method in the presence of 49% ammonium sulfate at 20 degrees C. The crystals are monoclinic and belong to the space group C2 with unit cell dimensions of a=49.18 A, b=93.08 A, c=82.10 A and beta=104.12 degrees. There was one molecule of alginate lyase in the asymmetric unit of the crystal. The diffraction data up to 1. 71 A were collected with Rsymof 5.0%. The crystal structure of ALYIII was solved by the multiple isomorphous replacement method and refined at 1.78 A resolution using X-PLOR with a final R -factor of 18.0% for 10.0 to 1.78 A resolution data. The refined model of ALYIII contained 351 amino acid residues, 299 water molecules and two sulfate ions. The three-dimensional structure of ALYIII was abundant in helices and had a deep tunnel-like cleft in a novel (alpha6/alpha5)-barrel structure, which was similar to the (alpha6/alpha6)-barrel found in glucoamylase and cellulase. This structure presented the possibility that alginate molecules might penetrate into the cleft to interact with the catalytic site of ALYIII.
About this Structure
1QAZ is a Single protein structure of sequence from Sphingomonas sp. with as ligand. Active as Urea carboxylase, with EC number 6.3.4.6 Full crystallographic information is available from OCA.
Reference
Crystal structure of alginate lyase A1-III from Sphingomonas species A1 at 1.78 A resolution., Yoon HJ, Mikami B, Hashimoto W, Murata K, J Mol Biol. 1999 Jul 9;290(2):505-14. PMID:10390348
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