1qbp
From Proteopedia
(New page: 200px<br /><applet load="1qbp" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qbp, resolution 2.1Å" /> '''CRYSTAL STRUCTURE OF ...) |
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| - | [[Image:1qbp.gif|left|200px]]<br /><applet load="1qbp" size=" | + | [[Image:1qbp.gif|left|200px]]<br /><applet load="1qbp" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qbp, resolution 2.1Å" /> | caption="1qbp, resolution 2.1Å" /> | ||
'''CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS'''<br /> | '''CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-ray crystal structure of a brominated RNA helix with four mismatched | + | The X-ray crystal structure of a brominated RNA helix with four mismatched base pairs and sequence r(UG(Br)C(Br)CAGUUCGCUGGC)(2) was determined to 2.1 A using the methods of multiwavelength anomalous diffraction (MAD) applied to the bromine K-absorption edge. There are three molecules in the asymmetric unit with unique crystal-packing environments, revealing true conformational variability at high resolution for this sequence. The structure shows that the sequence itself does not define a consistent pattern of solvent molecules, with the exception of the mismatched base pairs, implying that specific RNA-protein interactions would occur only with the nucleotides. There are a number of significant tertiary interactions, some of which are a result of the brominated base pairs and others that are directly mediated by the RNA 2' hydroxyl groups. The mismatched base pairs exhibit a solvent network as well as a stacking pattern with their nearest neighbors that validate previous thermodynamic analysis. |
==About this Structure== | ==About this Structure== | ||
| - | 1QBP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1QBP is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QBP OCA]. |
==Reference== | ==Reference== | ||
Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability., Anderson AC, O'Neil RH, Filman DJ, Frederick CA, Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10504226 10504226] | Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability., Anderson AC, O'Neil RH, Filman DJ, Frederick CA, Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10504226 10504226] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Anderson, A | + | [[Category: Anderson, A C.]] |
| - | [[Category: Filman, D | + | [[Category: Filman, D J.]] |
| - | [[Category: Frederick, C | + | [[Category: Frederick, C A.]] |
| - | [[Category: Neil, R | + | [[Category: Neil, R H.O.]] |
[[Category: brominated cytosine]] | [[Category: brominated cytosine]] | ||
[[Category: mismatched base pairs]] | [[Category: mismatched base pairs]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:37:57 2008'' |
Revision as of 12:37, 21 February 2008
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CRYSTAL STRUCTURE OF A BROMINATED RNA HELIX WITH FOUR MISMATCHED BASE PAIRS
Overview
The X-ray crystal structure of a brominated RNA helix with four mismatched base pairs and sequence r(UG(Br)C(Br)CAGUUCGCUGGC)(2) was determined to 2.1 A using the methods of multiwavelength anomalous diffraction (MAD) applied to the bromine K-absorption edge. There are three molecules in the asymmetric unit with unique crystal-packing environments, revealing true conformational variability at high resolution for this sequence. The structure shows that the sequence itself does not define a consistent pattern of solvent molecules, with the exception of the mismatched base pairs, implying that specific RNA-protein interactions would occur only with the nucleotides. There are a number of significant tertiary interactions, some of which are a result of the brominated base pairs and others that are directly mediated by the RNA 2' hydroxyl groups. The mismatched base pairs exhibit a solvent network as well as a stacking pattern with their nearest neighbors that validate previous thermodynamic analysis.
About this Structure
1QBP is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystal structure of a brominated RNA helix with four mismatched base pairs: An investigation into RNA conformational variability., Anderson AC, O'Neil RH, Filman DJ, Frederick CA, Biochemistry. 1999 Sep 28;38(39):12577-85. PMID:10504226
Page seeded by OCA on Thu Feb 21 14:37:57 2008
