Sandbox Reserved 654

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
('''Mechanism''')
(Symptoms)
Line 42: Line 42:
==== Symptoms ====
==== Symptoms ====
-
[[Image:PKU diet.jpg|thumb|300 px|right|PKU diet]]
+
<Structure load='1WUG' size='500' frame='true' align='right' caption='Insert caption here' scene='Insert optional scene name here' />
Phenylalanine plays a variety of roles in the body among which is the production of melanin, the pigment responsible for hair and skin color. Infants with an overabundance of this residue may therefore have a lighter skin, hair and eye color than those who do not. <ref> A.D.A.M Medical Encyclopedia, Phenylketonuria. [http://http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0002150/]</ref>
Phenylalanine plays a variety of roles in the body among which is the production of melanin, the pigment responsible for hair and skin color. Infants with an overabundance of this residue may therefore have a lighter skin, hair and eye color than those who do not. <ref> A.D.A.M Medical Encyclopedia, Phenylketonuria. [http://http://www.ncbi.nlm.nih.gov/pubmedhealth/PMH0002150/]</ref>

Revision as of 00:43, 18 November 2012

This Sandbox is Reserved from 30/08/2012, through 01/02/2013 for use in the course "Proteins and Molecular Mechanisms" taught by Robert B. Rose at the North Carolina State University, Raleigh, NC USA. This reservation includes Sandbox Reserved 636 through Sandbox Reserved 685.
To get started:
  • Click the edit this page tab at the top. Save the page after each step, then edit it again.
  • Click the 3D button (when editing, above the wikitext box) to insert Jmol.
  • show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page.
  • Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc.

More help: Help:Editing

For more help, look at this link: http://proteopedia.org/w/Help:Getting_Started_in_Proteopedia


Contents

p300/CBP-associated factor

This is a model of the pheylalanine hydroxylase dimer as found in humans. The green ball in within each subunit represents the iron ion in the catalytic domains.

Drag the structure with the mouse to rotate

p300/CBP-associated factor (PCAF), [1]

Structure

Structure stuff[2]

Catalytic Domain

The of phenylalanine hydroxylase includes resides 143-410. This region has a basket-like arrangement consisting of 13 alpha-helices and 8 beta-strands. This region of the protein also includes the active site. The active site of PheOH can be found in the center of the catalytic domain and is characterized by a 13 Angstroms deep and 10 Angstroms wide hydrophobic pocket. Lining the active site are 3 glutamates, 2 histadines and 1 tyrosine residue along with hydrophobic residues for a total of 34 amino acids. Covering the entrance of the active site is a short loop consisting or residues 378-381. The center of each catalytic domain consists of an iron ion which is vital to the enzyme activity. The iron atom binds in the active site to . Histadine 285 and 290 were found to be required for the binding of iron through site directed mutagenisis studies. The iron ions are coordinated to three water molecules and arrange in an octahedral geometry. The active site also binds the . This cofactor binds closely to the iron ion and forms hydrogen bonds with two of the three water molecules. The cofactor also forms hydrogen bonds with the carbonyl oxygen of the protein residues including Ala322, Gly247, and Leu249 and the amide of Leu249.[3]

Tetramerization Domain

Phenylalanine Hydroxylase exists in equilibrium between a homodimer and a homotetramer. The region responsible for the tertamerization is the located at the C terminal end of the protein. It consists of residues 411-452. The tetramerization domain consists of 2 beta-strands forming a beta-ribbon and an alpha-helix that is 40 angstroms long. The four alpha helices, consisting of one from each monomer, pack into a coil coil motif with the helices arranged in an anti-parallel manner.[4]

Regulatory Domain

Housed in the N-terminus, the regulatory domain contains residues 19-142 and is more flexible than the other domains. The core of this domain contains an alpha beta sandwich and a beta alpha beta double motif. [5]

Mechanism

Acetylation of the lysine and its effects on chromatin remodeling..
Acetylation of the lysine and its effects on chromatin remodeling..

The mechanism of protein-protein interaction for the bromodomain of PCAF with histone proteins begins with the acetylation of lysine residues on the histone tail. The acetylation causes a conformational change in the histones, which allows for transcriptional machinery to access DNA. The bromodomains of PCAF within a pretranscriptional initiation complex (PIC) bind to the acetyl-lysine of the histone to stabilize the complex so that transcription may begin. The bromodomains of PCAF have three major points of contact that allow for site-specific histone recognition. First, the acetylated lysine of the target protein enters a hydrophobic pocket embedded between the ZA and BC loops at the bottom of the protein. The Asn803 residue in the bromodomain forms a hydrogen bond with the amide nitrogen of the acetyl-lysine. Next, residues in the ZA and/or BC loops interact with residues adjacent to the acetyl-lysine, which reinforces the acetyl-lysine binding in the bromodomain. Finally, additional residues in the ZA and BC loops that face opposite to the bromodomain form hydrophobic and/ or electrostatic interaction with the target protein 3 residues away from the acetyl-lysine. This residue clamps on the BC loop together with the acetyl-lysine side chain that is bound inside the hydrophobic pocket of the bromodomain.[8]


The histone acetyltransferase portion of PCAF helps with the transactivation of HIV-1 by acetylating Lys28 of Tat. The acetylated Lys28 of Tat interacts with positive elongation factors, which stimulates elongation of nascent HIV-1 transcripts. [9]


The bromodomain of PCAF recognizes acetylated Lys50 on Tat, but not Lys28.

Implications or Possible Applications

The first diagnosed cases of Phenylketonuria (PKU), otherwise known as Folling's Disease, were identified in 1934 by Norwegian doctor and biochemist Asbjorn Folling. Dr. Folling found that the urine of two of his young mentally handicapped patients contained a high level of phenylalanine. Follwing this discovery, it was found that the absence or malfunction of the phenylalanine hydroxylase enzyme is due to the mutation of the PAH gene and inherited autosomal recessively. This may result in a genetic disorder known as Phenylketonuria (PKU). This information was not utilized until the early 1950s when it was found that under a low phenylalanine diet, some of the symptoms found in children suffering from PKU could be reversed. Due to a diet rich in phenylalanine, this enzyme is vital in the regulation in phenylalanine plasma concentration by converting about 75% of the amino acid to tyrosine. Excessive amounts of phenylalanine has been shown to cause mental retardation in humans. Presently, it is regulation to screen newborns children for phenylketonuria with a simple blood or urine test. [6] Due to his discovery and development of the PKU test, Dr. Folling is remembered as one of the most important medical scientists that has not received a Nobel Prize for Physiology or Medicine. [7]

Symptoms

Insert caption here

Drag the structure with the mouse to rotate

Phenylalanine plays a variety of roles in the body among which is the production of melanin, the pigment responsible for hair and skin color. Infants with an overabundance of this residue may therefore have a lighter skin, hair and eye color than those who do not. [8]

Other symptoms may include:

- Delayed mental and social skills

- Head size significantly below normal

- Hyperactivity

- Jerking movements of the arms or legs

- Mental retardation

- Seizures

- Skin rashes

- Tremors

- Unusual positioning of hands

Treatment

Treatment for such a PKU is a low phenylalanine diet and early detection. Those who start the diet early and adhere to it will have better mental and physical health. Infants diagnosed with the disease can fed a specially made formula called Lofenalac while others should follow a diet plan as illustrated in the image to the left. The main rule to follow is to avoid protein sources rich in phenylalanine and sugars containing aspartame. Taking extra supplements like fish oil can replace the fatty acids missing from the phenylalanine free diet and may also improve neurological development. PKU can also be caused by a deficiency in or inability to regenerate tetrahydrobipternin, the cofactor essential to the function of PheOH. Although this is not usually the cause of PKU, patients can be treated by taking tetrahydrobiopterin supplements. If the diet is not strictly followed, mental retardation may result after the first year of life. [9]


References

  1. sample ref
  2. sample ref
  3. Erlandsen H., DirSci; Marianne G. Patch, PhD; Alejandra Gamez, PhD; Mary Straub; and Raymond C. Stevens, PhD. Structural Studies on Phenylalanine Hydroxylase and Implications Toward Understanding and Treating Phenylketonuria [1]
  4. Erlandsen H., DirSci; Marianne G. Patch, PhD; Alejandra Gamez, PhD; Mary Straub; and Raymond C. Stevens, PhD. Structural Studies on Phenylalanine Hydroxylase and Implications Toward Understanding and Treating Phenylketonuria [2]
  5. Bostjan Kobe, Ian G. Jennings, Colin M. House1, Belinda J. Michell, Kenneth E. Goodwill, Bernard D. Santarsiero, Raymond C. Stevens, Richard G. H. Cotton and Bruce E. Kemp. Nature Structural Biology 6, 442 - 448 (1999), Structural basis of autoregulation of phenylalanine hydroxylase, [3]
  6. January 2005: Phenylalanine Hydroxylase [4]
  7. Dr. Ivar Asbjorn Folling- The Man Who discovered PKU Disorder [5]
  8. A.D.A.M Medical Encyclopedia, Phenylketonuria. [6]
  9. A.D.A.M Medical Encyclopedia, Phenylketonuria. [7]
Personal tools