1qcs
From Proteopedia
(New page: 200px<br /><applet load="1qcs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qcs, resolution 1.90Å" /> '''N-TERMINAL DOMAIN OF...) |
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| - | [[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size=" | + | [[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qcs, resolution 1.90Å" /> | caption="1qcs, resolution 1.90Å" /> | ||
'''N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br /> | '''N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential | + | N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly. |
==About this Structure== | ==About this Structure== | ||
| - | 1QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brunger, A | + | [[Category: Brunger, A T.]] |
[[Category: Jahn, R.]] | [[Category: Jahn, R.]] | ||
| - | [[Category: Yu, R | + | [[Category: Yu, R C.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: double-psi beta barrel alpha beta roll]] | [[Category: double-psi beta barrel alpha beta roll]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:38:20 2008'' |
Revision as of 12:38, 21 February 2008
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N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)
Overview
N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly.
About this Structure
1QCS is a Single protein structure of sequence from Cricetulus griseus with as ligand. Full crystallographic information is available from OCA.
Reference
NSF N-terminal domain crystal structure: models of NSF function., Yu RC, Jahn R, Brunger AT, Mol Cell. 1999 Jul;4(1):97-107. PMID:10445031
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