This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1qcs
From Proteopedia
(New page: 200px<br /><applet load="1qcs" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qcs, resolution 1.90Å" /> '''N-TERMINAL DOMAIN OF...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size=" | + | [[Image:1qcs.jpg|left|200px]]<br /><applet load="1qcs" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qcs, resolution 1.90Å" /> | caption="1qcs, resolution 1.90Å" /> | ||
'''N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br /> | '''N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential | + | N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly. |
==About this Structure== | ==About this Structure== | ||
| - | 1QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1QCS is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Cricetulus_griseus Cricetulus griseus] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QCS OCA]. |
==Reference== | ==Reference== | ||
| Line 13: | Line 13: | ||
[[Category: Cricetulus griseus]] | [[Category: Cricetulus griseus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brunger, A | + | [[Category: Brunger, A T.]] |
[[Category: Jahn, R.]] | [[Category: Jahn, R.]] | ||
| - | [[Category: Yu, R | + | [[Category: Yu, R C.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: double-psi beta barrel alpha beta roll]] | [[Category: double-psi beta barrel alpha beta roll]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:38:20 2008'' |
Revision as of 12:38, 21 February 2008
|
N-TERMINAL DOMAIN OF N-ETHYLMALEIMIDE SENSITIVE FACTOR (NSF)
Overview
N-ethylmaleimide-sensitive factor (NSF) is a hexameric ATPase essential for eukaryotic vesicle fusion. Along with SNAP proteins, it disassembles cis-SNARE complexes upon ATP hydrolysis, preparing SNAREs for trans complex formation. We have determined the crystal structure of the N-terminal domain of NSF (N) to 1.9 A resolution. N contains two subdomains which form a groove that is a likely SNAP interaction site. Unexpectedly, both N subdomains are structurally similar to domains in EF-Tu. Based on this similarity, we propose a model for a large conformational change in NSF that drives SNARE complex disassembly.
About this Structure
1QCS is a Single protein structure of sequence from Cricetulus griseus with as ligand. Full crystallographic information is available from OCA.
Reference
NSF N-terminal domain crystal structure: models of NSF function., Yu RC, Jahn R, Brunger AT, Mol Cell. 1999 Jul;4(1):97-107. PMID:10445031
Page seeded by OCA on Thu Feb 21 14:38:20 2008
