1qfx
From Proteopedia
(New page: 200px<br /><applet load="1qfx" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qfx, resolution 2.4Å" /> '''PH 2.5 ACID PHOSPHATA...) |
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| - | [[Image:1qfx.jpg|left|200px]]<br /><applet load="1qfx" size=" | + | [[Image:1qfx.jpg|left|200px]]<br /><applet load="1qfx" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qfx, resolution 2.4Å" /> | caption="1qfx, resolution 2.4Å" /> | ||
'''PH 2.5 ACID PHOSPHATASE FROM ASPERGILLUS NIGER'''<br /> | '''PH 2.5 ACID PHOSPHATASE FROM ASPERGILLUS NIGER'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystal structure of Aspergillus niger pH 2.5 acid phosphatase (EC | + | The crystal structure of Aspergillus niger pH 2.5 acid phosphatase (EC 3.1.3.2) has been determined at 2.4 A resolution. In the crystal, two dimers form a tetramer in which the active sites are easily accessible to substrates. The main contacts in the dimer come from the N termini, each lying on the surface of the neighbouring molecule. The monomer consists of two domains, with the active site located at their interface. The active site has a highly conserved catalytic center and a charge distribution, which explains the highly acidic pH optimum and the broad substrate specificity of the enzyme. |
==About this Structure== | ==About this Structure== | ||
| - | 1QFX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with NAG, SO4 and GOL as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] Full crystallographic information is available from [http:// | + | 1QFX is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger] with <scene name='pdbligand=NAG:'>NAG</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=GOL:'>GOL</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/3-phytase 3-phytase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.8 3.1.3.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QFX OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Aspergillus niger]] | [[Category: Aspergillus niger]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Arcy, A | + | [[Category: Arcy, A D.]] |
[[Category: Kostrewa, D.]] | [[Category: Kostrewa, D.]] | ||
| - | [[Category: Loon, A | + | [[Category: Loon, A P.G M.Van.]] |
[[Category: Wyss, M.]] | [[Category: Wyss, M.]] | ||
[[Category: GOL]] | [[Category: GOL]] | ||
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[[Category: phosphomonoesterase]] | [[Category: phosphomonoesterase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:06 2008'' |
Revision as of 12:39, 21 February 2008
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PH 2.5 ACID PHOSPHATASE FROM ASPERGILLUS NIGER
Overview
The crystal structure of Aspergillus niger pH 2.5 acid phosphatase (EC 3.1.3.2) has been determined at 2.4 A resolution. In the crystal, two dimers form a tetramer in which the active sites are easily accessible to substrates. The main contacts in the dimer come from the N termini, each lying on the surface of the neighbouring molecule. The monomer consists of two domains, with the active site located at their interface. The active site has a highly conserved catalytic center and a charge distribution, which explains the highly acidic pH optimum and the broad substrate specificity of the enzyme.
About this Structure
1QFX is a Single protein structure of sequence from Aspergillus niger with , and as ligands. Active as 3-phytase, with EC number 3.1.3.8 Full crystallographic information is available from OCA.
Reference
Crystal structure of Aspergillus niger pH 2.5 acid phosphatase at 2. 4 A resolution., Kostrewa D, Wyss M, D'Arcy A, van Loon AP, J Mol Biol. 1999 May 21;288(5):965-74. PMID:10329192
Page seeded by OCA on Thu Feb 21 14:39:06 2008
Categories: 3-phytase | Aspergillus niger | Single protein | Arcy, A D. | Kostrewa, D. | Loon, A P.G M.Van. | Wyss, M. | GOL | NAG | SO4 | Phosphomonoesterase
