This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1bza
From Proteopedia
| Line 29: | Line 29: | ||
[[Category: hydrolase]] | [[Category: hydrolase]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 14:58:22 2007'' |
Revision as of 12:53, 30 October 2007
|
BETA-LACTAMASE TOHO-1 FROM ESCHERICHIA COLI TUH12191
Overview
Bacterial resistance to beta-lactams is mainly due to the production of, beta-lactamase. Especially through the production of extended-spectrum, beta-lactamases (ESBLs), bacteria have acquired resistance not only to, penicillins, but also to expanded-spectrum cephems. Here, we describe the, crystal structure of the E166A mutant of class A beta-lactamase Toho-1 at, 1.8 A resolution, the first reported tertiary structure of an ESBL., Instead of the wild-type enzyme, a mutant Toho-1, in which Glu166 was, replaced with alanine, was used for this study, because of the strong, tendency of the wild-type enzyme to form twinned crystals. The overall, structure of Toho-1 is similar to the crystal structures of non-ESBLs, with no pronounced backbone rearrangement of the framework. However, there, ... [(full description)]
About this Structure
1BZA is a [Single protein] structure of sequence from [Escherichia coli] with SO4 as [ligand]. Active as [Beta-lactamase], with EC number [3.5.2.6]. Structure known Active Site: ACT. Full crystallographic information is available from [OCA].
Reference
Crystal structure of the E166A mutant of extended-spectrum beta-lactamase Toho-1 at 1.8 A resolution., Ibuka A, Taguchi A, Ishiguro M, Fushinobu S, Ishii Y, Kamitori S, Okuyama K, Yamaguchi K, Konno M, Matsuzawa H, J Mol Biol. 1999 Feb 5;285(5):2079-87. PMID:9925786
Page seeded by OCA on Tue Oct 30 14:58:22 2007
