1qgr
From Proteopedia
(New page: 200px<br /> <applet load="1qgr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qgr, resolution 2.3Å" /> '''STRUCTURE OF IMPORTI...) |
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| - | [[Image:1qgr.gif|left|200px]]<br /> | + | [[Image:1qgr.gif|left|200px]]<br /><applet load="1qgr" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1qgr" size=" | + | |
caption="1qgr, resolution 2.3Å" /> | caption="1qgr, resolution 2.3Å" /> | ||
'''STRUCTURE OF IMPORTIN BETA BOUND TO THE IBB DOMAIN OF IMPORTIN ALPHA (II CRYSTAL FORM, GROWN AT LOW PH)'''<br /> | '''STRUCTURE OF IMPORTIN BETA BOUND TO THE IBB DOMAIN OF IMPORTIN ALPHA (II CRYSTAL FORM, GROWN AT LOW PH)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Cytosolic proteins bearing a classical nuclear localization signal enter | + | Cytosolic proteins bearing a classical nuclear localization signal enter the nucleus bound to a heterodimer of importin-alpha and importin-beta (also called karyopherin-alpha and -beta). The formation of this heterodimer involves the importin-beta-binding (IBB) domain of importin-alpha, a highly basic amino-terminal region of roughly 40 amino-acid residues. Here we report the crystal structure of human importin-beta bound to the IBB domain of importin-alpha, determined at 2.5 A and 2.3 A resolution in two crystal forms. Importin-beta consists of 19 tandemly repeated HEAT motifs and wraps intimately around the IBB domain. The association involves two separate regions of importin-beta, recognizing structurally distinct parts of the IBB domain: an amino-terminal extended moiety and a carboxy-terminal helix. The structure indicates that significant conformational changes occur when importin-beta binds or releases the IBB domain domain and suggests how dissociation of the importin-alpha/beta heterodimer may be achieved upon nuclear entry. |
==About this Structure== | ==About this Structure== | ||
| - | 1QGR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1QGR is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QGR OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Cingolani, G.]] | [[Category: Cingolani, G.]] | ||
| - | [[Category: Muller, C | + | [[Category: Muller, C W.]] |
[[Category: Petosa, C.]] | [[Category: Petosa, C.]] | ||
[[Category: Weis, K.]] | [[Category: Weis, K.]] | ||
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[[Category: transport receptor]] | [[Category: transport receptor]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:23 2008'' |
Revision as of 12:39, 21 February 2008
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STRUCTURE OF IMPORTIN BETA BOUND TO THE IBB DOMAIN OF IMPORTIN ALPHA (II CRYSTAL FORM, GROWN AT LOW PH)
Overview
Cytosolic proteins bearing a classical nuclear localization signal enter the nucleus bound to a heterodimer of importin-alpha and importin-beta (also called karyopherin-alpha and -beta). The formation of this heterodimer involves the importin-beta-binding (IBB) domain of importin-alpha, a highly basic amino-terminal region of roughly 40 amino-acid residues. Here we report the crystal structure of human importin-beta bound to the IBB domain of importin-alpha, determined at 2.5 A and 2.3 A resolution in two crystal forms. Importin-beta consists of 19 tandemly repeated HEAT motifs and wraps intimately around the IBB domain. The association involves two separate regions of importin-beta, recognizing structurally distinct parts of the IBB domain: an amino-terminal extended moiety and a carboxy-terminal helix. The structure indicates that significant conformational changes occur when importin-beta binds or releases the IBB domain domain and suggests how dissociation of the importin-alpha/beta heterodimer may be achieved upon nuclear entry.
About this Structure
1QGR is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of importin-beta bound to the IBB domain of importin-alpha., Cingolani G, Petosa C, Weis K, Muller CW, Nature. 1999 May 20;399(6733):221-9. PMID:10353244
Page seeded by OCA on Thu Feb 21 14:39:23 2008
