1qhq
From Proteopedia
(New page: 200px<br /><applet load="1qhq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qhq, resolution 1.55Å" /> '''AURACYANIN, A BLUE C...) |
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| - | [[Image:1qhq.jpg|left|200px]]<br /><applet load="1qhq" size=" | + | [[Image:1qhq.jpg|left|200px]]<br /><applet load="1qhq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qhq, resolution 1.55Å" /> | caption="1qhq, resolution 1.55Å" /> | ||
'''AURACYANIN, A BLUE COPPER PROTEIN FROM THE GREEN THERMOPHILIC PHOTOSYNTHETIC BACTERIUM CHLOROFLEXUS AURANTIACUS'''<br /> | '''AURACYANIN, A BLUE COPPER PROTEIN FROM THE GREEN THERMOPHILIC PHOTOSYNTHETIC BACTERIUM CHLOROFLEXUS AURANTIACUS'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Auracyanin B, one of two similar blue copper proteins produced by the | + | Auracyanin B, one of two similar blue copper proteins produced by the thermophilic green non-sulfur photosynthetic bacterium Chloroflexus aurantiacus, crystallizes in space group P6(4)22 (a=b=115.7 A, c=54.6 A). The structure was solved using multiple wavelength anomalous dispersion data recorded about the CuK absorption edge, and was refined at 1.55 A resolution. The molecular model comprises 139 amino acid residues, one Cu, 247 H(2)O molecules, one Cl(-) and two SO(4)(2-). The final residual and estimated standard uncertainties are R=0.198, ESU=0.076 A for atomic coordinates and ESU=0.05 A for Cu---ligand bond lengths, respectively. The auracyanin B molecule has a standard cupredoxin fold. With the exception of an additional N-terminal strand, the molecule is very similar to that of the bacterial cupredoxin, azurin. As in other cupredoxins, one of the Cu ligands lies on strand 4 of the polypeptide, and the other three lie along a large loop between strands 7 and 8. The Cu site geometry is discussed with reference to the amino acid spacing between the latter three ligands. The crystallographically characterized Cu-binding domain of auracyanin B is probably tethered to the periplasmic side of the cytoplasmic membrane by an N-terminal tail that exhibits significant sequence identity with known tethers in several other membrane-associated electron-transfer proteins. |
==About this Structure== | ==About this Structure== | ||
| - | 1QHQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus] with CU, CL and SO4 as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1QHQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chloroflexus_aurantiacus Chloroflexus aurantiacus] with <scene name='pdbligand=CU:'>CU</scene>, <scene name='pdbligand=CL:'>CL</scene> and <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QHQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Chloroflexus aurantiacus]] | [[Category: Chloroflexus aurantiacus]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Blankenship, R | + | [[Category: Blankenship, R E.]] |
| - | [[Category: Bond, C | + | [[Category: Bond, C S.]] |
| - | [[Category: Freeman, H | + | [[Category: Freeman, H C.]] |
| - | [[Category: Guss, J | + | [[Category: Guss, J M.]] |
[[Category: Maher, M.]] | [[Category: Maher, M.]] | ||
[[Category: Selvaraj, F.]] | [[Category: Selvaraj, F.]] | ||
| - | [[Category: Wilce, M | + | [[Category: Wilce, M C.J.]] |
[[Category: Willingham, K.]] | [[Category: Willingham, K.]] | ||
[[Category: CL]] | [[Category: CL]] | ||
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[[Category: thermophile]] | [[Category: thermophile]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:42 2008'' |
Revision as of 12:39, 21 February 2008
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AURACYANIN, A BLUE COPPER PROTEIN FROM THE GREEN THERMOPHILIC PHOTOSYNTHETIC BACTERIUM CHLOROFLEXUS AURANTIACUS
Overview
Auracyanin B, one of two similar blue copper proteins produced by the thermophilic green non-sulfur photosynthetic bacterium Chloroflexus aurantiacus, crystallizes in space group P6(4)22 (a=b=115.7 A, c=54.6 A). The structure was solved using multiple wavelength anomalous dispersion data recorded about the CuK absorption edge, and was refined at 1.55 A resolution. The molecular model comprises 139 amino acid residues, one Cu, 247 H(2)O molecules, one Cl(-) and two SO(4)(2-). The final residual and estimated standard uncertainties are R=0.198, ESU=0.076 A for atomic coordinates and ESU=0.05 A for Cu---ligand bond lengths, respectively. The auracyanin B molecule has a standard cupredoxin fold. With the exception of an additional N-terminal strand, the molecule is very similar to that of the bacterial cupredoxin, azurin. As in other cupredoxins, one of the Cu ligands lies on strand 4 of the polypeptide, and the other three lie along a large loop between strands 7 and 8. The Cu site geometry is discussed with reference to the amino acid spacing between the latter three ligands. The crystallographically characterized Cu-binding domain of auracyanin B is probably tethered to the periplasmic side of the cytoplasmic membrane by an N-terminal tail that exhibits significant sequence identity with known tethers in several other membrane-associated electron-transfer proteins.
About this Structure
1QHQ is a Single protein structure of sequence from Chloroflexus aurantiacus with , and as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structure of auracyanin, a "blue" copper protein from the green thermophilic photosynthetic bacterium Chloroflexus aurantiacus., Bond CS, Blankenship RE, Freeman HC, Guss JM, Maher MJ, Selvaraj FM, Wilce MC, Willingham KM, J Mol Biol. 2001 Feb 9;306(1):47-67. PMID:11178893
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