1qhv
From Proteopedia
(New page: 200px<br /><applet load="1qhv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qhv, resolution 1.51Å" /> '''HUMAN ADENOVIRUS SER...) |
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| - | [[Image:1qhv.jpg|left|200px]]<br /><applet load="1qhv" size=" | + | [[Image:1qhv.jpg|left|200px]]<br /><applet load="1qhv" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qhv, resolution 1.51Å" /> | caption="1qhv, resolution 1.51Å" /> | ||
'''HUMAN ADENOVIRUS SEROTYPE 2 FIBRE HEAD'''<br /> | '''HUMAN ADENOVIRUS SEROTYPE 2 FIBRE HEAD'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Adenovirus binds to its receptor via the head domain of its fiber protein. | + | Adenovirus binds to its receptor via the head domain of its fiber protein. We have crystallized the adenovirus serotype 2 (subgroup C) receptor binding domain and solved the structure at 1.5 A resolution by the molecular replacement technique using the known adenovirus type 5 head structure. Included in the high-resolution model are 306 water molecules, five alternative side chain conformations, and individual anisotropic temperature factors for each atom. The overall structure of the serotype 2 head is very similar to its serotype 5 homologue, apart from differences in some of the flexible loops. All but subgroup B adenoviruses are believed to use the recently identified protein CAR (Coxsackievirus and adenovirus receptor) as receptor. By comparison of the two structures and sequence alignment of CAR binding and non-CAR binding serotype fiber heads, we discuss possible receptor binding sites and propose a receptor binding site in a crevice between two monomers on the side of the trimer. The structural basis of the extraordinary stability of the fiber head trimer is also discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1QHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_2 Human adenovirus 2] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1QHV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Human_adenovirus_2 Human adenovirus 2] with <scene name='pdbligand=SO4:'>SO4</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QHV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Cusack, S.]] | [[Category: Cusack, S.]] | ||
[[Category: Louis, N.]] | [[Category: Louis, N.]] | ||
| - | [[Category: Raaij, M | + | [[Category: Raaij, M J.Van.]] |
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: extra-ordinary stability]] | [[Category: extra-ordinary stability]] | ||
[[Category: receptor binding]] | [[Category: receptor binding]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:39:44 2008'' |
Revision as of 12:39, 21 February 2008
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HUMAN ADENOVIRUS SEROTYPE 2 FIBRE HEAD
Overview
Adenovirus binds to its receptor via the head domain of its fiber protein. We have crystallized the adenovirus serotype 2 (subgroup C) receptor binding domain and solved the structure at 1.5 A resolution by the molecular replacement technique using the known adenovirus type 5 head structure. Included in the high-resolution model are 306 water molecules, five alternative side chain conformations, and individual anisotropic temperature factors for each atom. The overall structure of the serotype 2 head is very similar to its serotype 5 homologue, apart from differences in some of the flexible loops. All but subgroup B adenoviruses are believed to use the recently identified protein CAR (Coxsackievirus and adenovirus receptor) as receptor. By comparison of the two structures and sequence alignment of CAR binding and non-CAR binding serotype fiber heads, we discuss possible receptor binding sites and propose a receptor binding site in a crevice between two monomers on the side of the trimer. The structural basis of the extraordinary stability of the fiber head trimer is also discussed.
About this Structure
1QHV is a Single protein structure of sequence from Human adenovirus 2 with as ligand. Full crystallographic information is available from OCA.
Reference
Structure of the human adenovirus serotype 2 fiber head domain at 1.5 A resolution., van Raaij MJ, Louis N, Chroboczek J, Cusack S, Virology. 1999 Sep 30;262(2):333-43. PMID:10502512
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