1qld
From Proteopedia
(New page: 200px<br /><applet load="1qld" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qld" /> '''SOLUTION STRUCTURE OF TYPE X CBM'''<br /> =...) |
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'''SOLUTION STRUCTURE OF TYPE X CBM'''<br /> | '''SOLUTION STRUCTURE OF TYPE X CBM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Plant cell wall hydrolases generally have a modular structure consisting | + | Plant cell wall hydrolases generally have a modular structure consisting of a catalytic domain linked to one or more noncatalytic carbohydrate-binding modules (CBMs), whose common function is to attach the enzyme to the polymeric substrate. Xylanase A from Pseudomonas fluorescens subsp. cellulosa (Pf Xyn10A) consists of a family 10 catalytic domain, an N-terminal family IIa cellulose-binding module, and an internal family 10 cellulose-binding module. The structure of the 45-residue family 10 CBM has been determined in solution using NMR. It consists of two antiparallel beta-sheets, one with two strands and one with three, with a short alpha-helix across one face of the three-stranded sheet. There is a high density of aromatic residues on one side of the protein, including three aromatic residues (Tyr8, Trp22, and Trp24), which are exposed and form a flat surface on one face, in a classical polysaccharide-binding arrangement. The fold is closely similar to that of the oligonucleotide/oligosaccharide-binding (OB) fold, but appears to have arisen by convergent evolution, because there is no sequence similarity, and the presumed binding sites are on different faces. |
==About this Structure== | ==About this Structure== | ||
| - | 1QLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http:// | + | 1QLD is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens]. Active as [http://en.wikipedia.org/wiki/Endo-1,4-beta-xylanase Endo-1,4-beta-xylanase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.8 3.2.1.8] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QLD OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Pseudomonas fluorescens]] | [[Category: Pseudomonas fluorescens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Gilbert, H | + | [[Category: Gilbert, H J.]] |
[[Category: Raghothama, S.]] | [[Category: Raghothama, S.]] | ||
| - | [[Category: Simpson, P | + | [[Category: Simpson, P J.]] |
| - | [[Category: Williamson, M | + | [[Category: Williamson, M P.]] |
[[Category: anti parallel beta sheets]] | [[Category: anti parallel beta sheets]] | ||
[[Category: beta strands]] | [[Category: beta strands]] | ||
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[[Category: xylan degradation]] | [[Category: xylan degradation]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:40:54 2008'' |
Revision as of 12:40, 21 February 2008
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SOLUTION STRUCTURE OF TYPE X CBM
Overview
Plant cell wall hydrolases generally have a modular structure consisting of a catalytic domain linked to one or more noncatalytic carbohydrate-binding modules (CBMs), whose common function is to attach the enzyme to the polymeric substrate. Xylanase A from Pseudomonas fluorescens subsp. cellulosa (Pf Xyn10A) consists of a family 10 catalytic domain, an N-terminal family IIa cellulose-binding module, and an internal family 10 cellulose-binding module. The structure of the 45-residue family 10 CBM has been determined in solution using NMR. It consists of two antiparallel beta-sheets, one with two strands and one with three, with a short alpha-helix across one face of the three-stranded sheet. There is a high density of aromatic residues on one side of the protein, including three aromatic residues (Tyr8, Trp22, and Trp24), which are exposed and form a flat surface on one face, in a classical polysaccharide-binding arrangement. The fold is closely similar to that of the oligonucleotide/oligosaccharide-binding (OB) fold, but appears to have arisen by convergent evolution, because there is no sequence similarity, and the presumed binding sites are on different faces.
About this Structure
1QLD is a Single protein structure of sequence from Pseudomonas fluorescens. Active as Endo-1,4-beta-xylanase, with EC number 3.2.1.8 Full crystallographic information is available from OCA.
Reference
Solution structure of the CBM10 cellulose binding module from Pseudomonas xylanase A., Raghothama S, Simpson PJ, Szabo L, Nagy T, Gilbert HJ, Williamson MP, Biochemistry. 2000 Feb 8;39(5):978-84. PMID:10653641
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