1qo0
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Inducible expression of the aliphatic amidase operon in Pseudomonas | + | Inducible expression of the aliphatic amidase operon in Pseudomonas aeruginosa is controlled by an antitermination mechanism which allows production of the full-length transcript only in the presence of small-molecule inducers, such as acetamide. Ligand-regulated antitermination is provided by AmiC, the ligand-sensitive negative regulator, and AmiR, the RNA-binding positive regulator. Under non-inducing or repressing growth conditions, AmiC and AmiR form a complex in which the activity of AmiR is silenced. The crystal structure of the AmiC-AmiR complex identifies AmiR as a new and highly unusual member of the response-regulator family of bacterial signal transduction proteins, regulated by sequestration rather than phosphorylation. Comparison with the structure of free AmiC reveals the subtle mechanism of ligand-induced release of AmiR. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Pseudomonas aeruginosa]] | [[Category: Pseudomonas aeruginosa]] | ||
| - | [[Category: Hara, B | + | [[Category: Hara, B P.O.]] |
| - | [[Category: Pearl, L | + | [[Category: Pearl, L H.]] |
| - | [[Category: Roe, S | + | [[Category: Roe, S M.]] |
[[Category: BMD]] | [[Category: BMD]] | ||
[[Category: binding protein]] | [[Category: binding protein]] | ||
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[[Category: receptor]] | [[Category: receptor]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:41:49 2008'' |
Revision as of 12:41, 21 February 2008
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AMIDE RECEPTOR OF THE AMIDASE OPERON OF PSEUDOMONAS AERUGINOSA (AMIC) COMPLEXED WITH THE NEGATIVE REGULATOR AMIR.
Overview
Inducible expression of the aliphatic amidase operon in Pseudomonas aeruginosa is controlled by an antitermination mechanism which allows production of the full-length transcript only in the presence of small-molecule inducers, such as acetamide. Ligand-regulated antitermination is provided by AmiC, the ligand-sensitive negative regulator, and AmiR, the RNA-binding positive regulator. Under non-inducing or repressing growth conditions, AmiC and AmiR form a complex in which the activity of AmiR is silenced. The crystal structure of the AmiC-AmiR complex identifies AmiR as a new and highly unusual member of the response-regulator family of bacterial signal transduction proteins, regulated by sequestration rather than phosphorylation. Comparison with the structure of free AmiC reveals the subtle mechanism of ligand-induced release of AmiR.
About this Structure
1QO0 is a Protein complex structure of sequences from Pseudomonas aeruginosa with as ligand. Known structural/functional Sites: and . Full crystallographic information is available from OCA.
Reference
Crystal structure and induction mechanism of AmiC-AmiR: a ligand-regulated transcription antitermination complex., O'Hara BP, Norman RA, Wan PT, Roe SM, Barrett TE, Drew RE, Pearl LH, EMBO J. 1999 Oct 1;18(19):5175-86. PMID:10508151
Page seeded by OCA on Thu Feb 21 14:41:49 2008
