1qp5
From Proteopedia
(New page: 200px<br /><applet load="1qp5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qp5, resolution 2.6Å" /> '''BASE-PAIRING SHIFT IN...) |
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| - | [[Image:1qp5.gif|left|200px]]<br /><applet load="1qp5" size=" | + | [[Image:1qp5.gif|left|200px]]<br /><applet load="1qp5" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qp5, resolution 2.6Å" /> | caption="1qp5, resolution 2.6Å" /> | ||
'''BASE-PAIRING SHIFT IN A DODECAMER CONTAINING A (CA)N TRACT'''<br /> | '''BASE-PAIRING SHIFT IN A DODECAMER CONTAINING A (CA)N TRACT'''<br /> | ||
==Overview== | ==Overview== | ||
| - | the crystal packing of the B-DNA dodecamer | + | the crystal packing of the B-DNA dodecamer d(ACCG-GCGCCACA).d(TGTGGCGCCGGT) is characterized by the reciprocal fit of double helices with specific base-backbone interactions in the major groove. Cooling the crystals below -10 degrees C stabilizes a new conformational state with a long-range sequence-dependent one-step shift in the major-groove base pairing. The tilt of the bases leads to the disruption of the Watson-Crick pairing in the major groove and to the formation of interactions with the 5' neighbour of their complement. This alteration propagates along the helical axis over more than half a turn. As a result, the molecular structure is normal when seen from the minor groove side and mismatched in the major groove. Comparison with a parent isomorphous dodecamer structure corresponding to the codon 10-13 of the c-Ha-ras proto-oncogene show that this new structural feature is sequence dependent and clearly favoured by (CA)n tracts. As(CA)n tracts of DNA are involved both in recombination and in transcription, this new recognition pattern should be considered in the analysis of the various processes involving the reading of the genetic information. |
==About this Structure== | ==About this Structure== | ||
| - | 1QP5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1QP5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QP5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: microsatellite instability]] | [[Category: microsatellite instability]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:42:12 2008'' |
Revision as of 12:42, 21 February 2008
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BASE-PAIRING SHIFT IN A DODECAMER CONTAINING A (CA)N TRACT
Overview
the crystal packing of the B-DNA dodecamer d(ACCG-GCGCCACA).d(TGTGGCGCCGGT) is characterized by the reciprocal fit of double helices with specific base-backbone interactions in the major groove. Cooling the crystals below -10 degrees C stabilizes a new conformational state with a long-range sequence-dependent one-step shift in the major-groove base pairing. The tilt of the bases leads to the disruption of the Watson-Crick pairing in the major groove and to the formation of interactions with the 5' neighbour of their complement. This alteration propagates along the helical axis over more than half a turn. As a result, the molecular structure is normal when seen from the minor groove side and mismatched in the major groove. Comparison with a parent isomorphous dodecamer structure corresponding to the codon 10-13 of the c-Ha-ras proto-oncogene show that this new structural feature is sequence dependent and clearly favoured by (CA)n tracts. As(CA)n tracts of DNA are involved both in recombination and in transcription, this new recognition pattern should be considered in the analysis of the various processes involving the reading of the genetic information.
About this Structure
1QP5 is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Base-pairing shift in the major groove of (CA)n tracts by B-DNA crystal structures., Timsit Y, Vilbois E, Moras D, Nature. 1991 Nov 14;354(6349):167-70. PMID:1944598
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