1qqf
From Proteopedia
(New page: 200px<br /><applet load="1qqf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qqf, resolution 1.45Å" /> '''N-TERMINALLY TRUNCAT...) |
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| - | [[Image:1qqf.gif|left|200px]]<br /><applet load="1qqf" size=" | + | [[Image:1qqf.gif|left|200px]]<br /><applet load="1qqf" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qqf, resolution 1.45Å" /> | caption="1qqf, resolution 1.45Å" /> | ||
'''N-TERMINALLY TRUNCATED C3D,G FRAGMENT OF THE COMPLEMENT SYSTEM'''<br /> | '''N-TERMINALLY TRUNCATED C3D,G FRAGMENT OF THE COMPLEMENT SYSTEM'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Complement component C3 plays a key role in the complement-mediated immune | + | Complement component C3 plays a key role in the complement-mediated immune defence, and occupies a central position within the complement cascade system. One of its degradation products, C3dg, was purified from rat serum and crystallised in two different crystal forms as N-terminally truncated fragment. Despite the truncation and the lack of a significant portion of the N-terminus as compared to C3d, the structure of the fragment is highly similar to that of recombinant human C3d (Nagar et al., Science 280 (1998) 1277-1281). Structural details of the reactive site have been obtained, suggesting a possible mode of thioester bond formation between Cys-1010 and Gln-1013 and thioester bond cleavage in the transacylation reaction involving His-1126. The truncation at the N-terminus of C3d leads to the exposure of a surface of the molecule that favours dimerisation, so that in both crystal forms, the fragment is present as a dimer, with monomers related by a two-fold axis. |
==About this Structure== | ==About this Structure== | ||
| - | 1QQF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http:// | + | 1QQF is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QQF OCA]. |
==Reference== | ==Reference== | ||
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[[Category: complement]] | [[Category: complement]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:42:32 2008'' |
Revision as of 12:42, 21 February 2008
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N-TERMINALLY TRUNCATED C3D,G FRAGMENT OF THE COMPLEMENT SYSTEM
Overview
Complement component C3 plays a key role in the complement-mediated immune defence, and occupies a central position within the complement cascade system. One of its degradation products, C3dg, was purified from rat serum and crystallised in two different crystal forms as N-terminally truncated fragment. Despite the truncation and the lack of a significant portion of the N-terminus as compared to C3d, the structure of the fragment is highly similar to that of recombinant human C3d (Nagar et al., Science 280 (1998) 1277-1281). Structural details of the reactive site have been obtained, suggesting a possible mode of thioester bond formation between Cys-1010 and Gln-1013 and thioester bond cleavage in the transacylation reaction involving His-1126. The truncation at the N-terminus of C3d leads to the exposure of a surface of the molecule that favours dimerisation, so that in both crystal forms, the fragment is present as a dimer, with monomers related by a two-fold axis.
About this Structure
1QQF is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.
Reference
Structure at 1.44 A resolution of an N-terminally truncated form of the rat serum complement C3d fragment., Zanotti G, Bassetto A, Battistutta R, Folli C, Arcidiaco P, Stoppini M, Berni R, Biochim Biophys Acta. 2000 May 23;1478(2):232-8. PMID:10825534
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