1qqt
From Proteopedia
(New page: 200px<br /><applet load="1qqt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qqt, resolution 2.03Å" /> '''METHIONYL-TRNA SYNTH...) |
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| - | [[Image:1qqt.gif|left|200px]]<br /><applet load="1qqt" size=" | + | [[Image:1qqt.gif|left|200px]]<br /><applet load="1qqt" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qqt, resolution 2.03Å" /> | caption="1qqt, resolution 2.03Å" /> | ||
'''METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI'''<br /> | '''METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The 3D structure of monomeric C-truncated Escherichia coli methionyl-tRNA | + | The 3D structure of monomeric C-truncated Escherichia coli methionyl-tRNA synthetase, a class 1 aminoacyl-tRNA synthetase, has been solved at 2.0 A resolution. Remarkably, the polypeptide connecting the two halves of the Rossmann fold exposes two identical knuckles related by a 2-fold axis but with zinc in the distal knuckle only. Examination of available MetRS orthologs reveals four classes according to the number and zinc content of the putative knuckles. Extreme cases are exemplified by the MetRS of eucaryotic or archaeal origin, where two knuckles and two metal ions are expected, and by the mitochondrial enzymes, which are predicted to have one knuckle without metal ion. |
==About this Structure== | ==About this Structure== | ||
| - | 1QQT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Methionine--tRNA_ligase Methionine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.10 6.1.1.10] Full crystallographic information is available from [http:// | + | 1QQT is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Active as [http://en.wikipedia.org/wiki/Methionine--tRNA_ligase Methionine--tRNA ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.1.1.10 6.1.1.10] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QQT OCA]. |
==Reference== | ==Reference== | ||
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[[Category: trna ligase]] | [[Category: trna ligase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:42:40 2008'' |
Revision as of 12:42, 21 February 2008
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METHIONYL-TRNA SYNTHETASE FROM ESCHERICHIA COLI
Overview
The 3D structure of monomeric C-truncated Escherichia coli methionyl-tRNA synthetase, a class 1 aminoacyl-tRNA synthetase, has been solved at 2.0 A resolution. Remarkably, the polypeptide connecting the two halves of the Rossmann fold exposes two identical knuckles related by a 2-fold axis but with zinc in the distal knuckle only. Examination of available MetRS orthologs reveals four classes according to the number and zinc content of the putative knuckles. Extreme cases are exemplified by the MetRS of eucaryotic or archaeal origin, where two knuckles and two metal ions are expected, and by the mitochondrial enzymes, which are predicted to have one knuckle without metal ion.
About this Structure
1QQT is a Single protein structure of sequence from Escherichia coli with as ligand. Active as Methionine--tRNA ligase, with EC number 6.1.1.10 Full crystallographic information is available from OCA.
Reference
Crystal structure of Escherichia coli methionyl-tRNA synthetase highlights species-specific features., Mechulam Y, Schmitt E, Maveyraud L, Zelwer C, Nureki O, Yokoyama S, Konno M, Blanquet S, J Mol Biol. 1999 Dec 17;294(5):1287-97. PMID:10600385
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