1qvc
From Proteopedia
(New page: 200px<br /><applet load="1qvc" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qvc, resolution 2.2Å" /> '''CRYSTAL STRUCTURE ANA...) |
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| - | [[Image:1qvc.gif|left|200px]]<br /><applet load="1qvc" size=" | + | [[Image:1qvc.gif|left|200px]]<br /><applet load="1qvc" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qvc, resolution 2.2Å" /> | caption="1qvc, resolution 2.2Å" /> | ||
'''CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI'''<br /> | '''CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The single-stranded DNA (ssDNA) binding protein from Escherichia coli | + | The single-stranded DNA (ssDNA) binding protein from Escherichia coli (EcoSSB) plays a central role in DNA replication, recombination and repair. The tertiary structure of EcoSSB was determined at 2.2 A resolution. This is rather higher resolution than previously reported. Crystals were grown from the homogeneous intact protein but the EcoSSB tetramer in the crystals contains truncated subunits lacking a part of the C-terminal. The structure determined includes biologically important flexible loops and C-terminal regions, and revealed the existence of concavities. These concavities include the residues important for ssDNA binding. An ssDNA can be fitted on the concavities and further stabilized through interactions with the loops forming flexible lids. It seems likely to play a central role in the binding of ssDNA. |
==About this Structure== | ==About this Structure== | ||
| - | 1QVC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1QVC is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QVC OCA]. |
==Reference== | ==Reference== | ||
| - | Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis., Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N, J Biochem | + | Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis., Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N, J Biochem. 2000 Feb;127(2):329-35. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10731701 10731701] |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
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[[Category: single stranded dna binding protein]] | [[Category: single stranded dna binding protein]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:09 2008'' |
Revision as of 12:44, 21 February 2008
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CRYSTAL STRUCTURE ANALYSIS OF SINGLE STRANDED DNA BINDING PROTEIN (SSB) FROM E.COLI
Overview
The single-stranded DNA (ssDNA) binding protein from Escherichia coli (EcoSSB) plays a central role in DNA replication, recombination and repair. The tertiary structure of EcoSSB was determined at 2.2 A resolution. This is rather higher resolution than previously reported. Crystals were grown from the homogeneous intact protein but the EcoSSB tetramer in the crystals contains truncated subunits lacking a part of the C-terminal. The structure determined includes biologically important flexible loops and C-terminal regions, and revealed the existence of concavities. These concavities include the residues important for ssDNA binding. An ssDNA can be fitted on the concavities and further stabilized through interactions with the loops forming flexible lids. It seems likely to play a central role in the binding of ssDNA.
About this Structure
1QVC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Roles of functional loops and the C-terminal segment of a single-stranded DNA binding protein elucidated by X-Ray structure analysis., Matsumoto T, Morimoto Y, Shibata N, Kinebuchi T, Shimamoto N, Tsukihara T, Yasuoka N, J Biochem. 2000 Feb;127(2):329-35. PMID:10731701
Page seeded by OCA on Thu Feb 21 14:44:09 2008
