1qwi
From Proteopedia
(New page: 200px<br /><applet load="1qwi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qwi, resolution 1.8Å" /> '''Crystal Structure of ...) |
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| - | [[Image:1qwi.jpg|left|200px]]<br /><applet load="1qwi" size=" | + | [[Image:1qwi.jpg|left|200px]]<br /><applet load="1qwi" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qwi, resolution 1.8Å" /> | caption="1qwi, resolution 1.8Å" /> | ||
'''Crystal Structure of E. coli OsmC'''<br /> | '''Crystal Structure of E. coli OsmC'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The osmotically inducible protein OsmC, like its better-characterized | + | The osmotically inducible protein OsmC, like its better-characterized homolog, the organic hydroperoxide protein Ohr, is involved in defense against oxidative stress caused by exposure to organic hydroperoxides. The crystal structure of Escherichia coli OsmC reported here reveals that the protein is a tightly folded domain-swapped dimer with two active sites located at the monomer interface on opposite sides of the molecule. We demonstrate that OsmC preferentially metabolizes organic hydroperoxides over inorganic hydrogen peroxide. On the basis of structural and enzymatic similarities, we propose that the OsmC catalytic mechanism is analogous to that of the Ohr proteins and of the structurally unrelated peroxiredoxins, directly using highly reactive cysteine thiol groups to elicit hydroperoxide reduction. |
==About this Structure== | ==About this Structure== | ||
| - | 1QWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http:// | + | 1QWI is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWI OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Barton, W | + | [[Category: Barton, W A.]] |
[[Category: Lesniak, J.]] | [[Category: Lesniak, J.]] | ||
| - | [[Category: Nikolov, D | + | [[Category: Nikolov, D B.]] |
[[Category: hydroperoxide resistance]] | [[Category: hydroperoxide resistance]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:32 2008'' |
Revision as of 12:44, 21 February 2008
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Crystal Structure of E. coli OsmC
Overview
The osmotically inducible protein OsmC, like its better-characterized homolog, the organic hydroperoxide protein Ohr, is involved in defense against oxidative stress caused by exposure to organic hydroperoxides. The crystal structure of Escherichia coli OsmC reported here reveals that the protein is a tightly folded domain-swapped dimer with two active sites located at the monomer interface on opposite sides of the molecule. We demonstrate that OsmC preferentially metabolizes organic hydroperoxides over inorganic hydrogen peroxide. On the basis of structural and enzymatic similarities, we propose that the OsmC catalytic mechanism is analogous to that of the Ohr proteins and of the structurally unrelated peroxiredoxins, directly using highly reactive cysteine thiol groups to elicit hydroperoxide reduction.
About this Structure
1QWI is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structural and functional features of the Escherichia coli hydroperoxide resistance protein OsmC., Lesniak J, Barton WA, Nikolov DB, Protein Sci. 2003 Dec;12(12):2838-43. PMID:14627744
Page seeded by OCA on Thu Feb 21 14:44:32 2008
