1qwz
From Proteopedia
(New page: 200px<br /><applet load="1qwz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qwz, resolution 1.75Å" /> '''Crystal structure of...) |
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| - | [[Image:1qwz.jpg|left|200px]]<br /><applet load="1qwz" size=" | + | [[Image:1qwz.jpg|left|200px]]<br /><applet load="1qwz" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qwz, resolution 1.75Å" /> | caption="1qwz, resolution 1.75Å" /> | ||
'''Crystal structure of Sortase B from S. aureus complexed with MTSET'''<br /> | '''Crystal structure of Sortase B from S. aureus complexed with MTSET'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Many surface proteins of Gram-positive bacteria, which play important | + | Many surface proteins of Gram-positive bacteria, which play important roles during the pathogenesis of human infections, are anchored to the cell wall envelope by a mechanism requiring sortases. Sortase B, a cysteine transpeptidase from Staphylococcus aureus, cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and tethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges. We report here the crystal structures of SrtBDeltaN30 in complex with two active site inhibitors, MTSET and E64, and with the cell wall substrate analog tripleglycine. These structures reveal, for the first time, the active site disposition and the unique Cys-Arg catalytic machinery of the cysteine transpeptidase, and they also provide useful information for the future design of anti-infective agents against sortases. |
==About this Structure== | ==About this Structure== | ||
| - | 1QWZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with NI, SO4 and ETM as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1QWZ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus] with <scene name='pdbligand=NI:'>NI</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=ETM:'>ETM</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QWZ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus aureus]] | [[Category: Staphylococcus aureus]] | ||
| - | [[Category: Mazmanian, S | + | [[Category: Mazmanian, S K.]] |
| - | [[Category: Narayana, S | + | [[Category: Narayana, S V.]] |
[[Category: Schneewind, O.]] | [[Category: Schneewind, O.]] | ||
[[Category: Zong, Y.]] | [[Category: Zong, Y.]] | ||
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[[Category: transpeptidase]] | [[Category: transpeptidase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:37 2008'' |
Revision as of 12:44, 21 February 2008
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Crystal structure of Sortase B from S. aureus complexed with MTSET
Overview
Many surface proteins of Gram-positive bacteria, which play important roles during the pathogenesis of human infections, are anchored to the cell wall envelope by a mechanism requiring sortases. Sortase B, a cysteine transpeptidase from Staphylococcus aureus, cleaves the C-terminal sorting signal of IsdC at the NPQTN motif and tethers the polypeptide to the pentaglycine cell wall cross-bridge. During catalysis, the active site cysteine of sortase and the cleaved substrate form an acyl intermediate, which is then resolved by the amino group of pentaglycine cross-bridges. We report here the crystal structures of SrtBDeltaN30 in complex with two active site inhibitors, MTSET and E64, and with the cell wall substrate analog tripleglycine. These structures reveal, for the first time, the active site disposition and the unique Cys-Arg catalytic machinery of the cysteine transpeptidase, and they also provide useful information for the future design of anti-infective agents against sortases.
About this Structure
1QWZ is a Single protein structure of sequence from Staphylococcus aureus with , and as ligands. Full crystallographic information is available from OCA.
Reference
The structure of sortase B, a cysteine transpeptidase that tethers surface protein to the Staphylococcus aureus cell wall., Zong Y, Mazmanian SK, Schneewind O, Narayana SV, Structure. 2004 Jan;12(1):105-12. PMID:14725770
Page seeded by OCA on Thu Feb 21 14:44:37 2008
