1qxb
From Proteopedia
(New page: 200px<br /><applet load="1qxb" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qxb" /> '''NMR structure determination of the self comp...) |
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'''NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9'''<br /> | '''NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9'''<br /> | ||
==Overview== | ==Overview== | ||
| - | A ribose residue inserted between the 3'-OH of one nucleotide and the | + | A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information. |
==About this Structure== | ==About this Structure== | ||
| - | 1QXB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with RIB as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1QXB is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=RIB:'>RIB</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QXB OCA]. |
==Reference== | ==Reference== | ||
Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside., Nauwelaerts K, Vastmans K, Froeyen M, Kempeneers V, Rozenski J, Rosemeyer H, Van Aerschot A, Busson R, Lacey JC, Efimtseva E, Mikhailov S, Lescrinier E, Herdewijn P, Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14627809 14627809] | Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside., Nauwelaerts K, Vastmans K, Froeyen M, Kempeneers V, Rozenski J, Rosemeyer H, Van Aerschot A, Busson R, Lacey JC, Efimtseva E, Mikhailov S, Lescrinier E, Herdewijn P, Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14627809 14627809] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Aerschot, A | + | [[Category: Aerschot, A Van.]] |
[[Category: Busson, R.]] | [[Category: Busson, R.]] | ||
[[Category: Efimtseva, E.]] | [[Category: Efimtseva, E.]] | ||
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[[Category: ribose]] | [[Category: ribose]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:44:42 2008'' |
Revision as of 12:44, 21 February 2008
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NMR structure determination of the self complementary DNA Dodecamer CGCGAATT*CGCG in which a ribose is inserted between the 3'-OH of T8 and the 5'-phosphate group of C9
Overview
A ribose residue inserted between the 3'-OH of one nucleotide and the 5'-phosphate group of the next nucleotide, functions as a site-specific cleavage site within DNA. This extra ribose does not interrupt helix formation and it protects duplex DNA against cleavage by restriction enzymes. Cleavage can be obtained with periodate and all ribose fragments can be removed with sodium hydroxide. As a result of this, an intact natural oligodeoxynucleotide is obtained after ligation reaction, which means that site-specific cleavage and recovering of intact DNA occurs without loss of genetic information.
About this Structure
1QXB is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
Cleavage of DNA without loss of genetic information by incorporation of a disaccharide nucleoside., Nauwelaerts K, Vastmans K, Froeyen M, Kempeneers V, Rozenski J, Rosemeyer H, Van Aerschot A, Busson R, Lacey JC, Efimtseva E, Mikhailov S, Lescrinier E, Herdewijn P, Nucleic Acids Res. 2003 Dec 1;31(23):6758-69. PMID:14627809
Page seeded by OCA on Thu Feb 21 14:44:42 2008
