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1qz9
From Proteopedia
(New page: 200px<br /><applet load="1qz9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qz9, resolution 1.85Å" /> '''The Three Dimensiona...) |
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| - | [[Image:1qz9.jpg|left|200px]]<br /><applet load="1qz9" size=" | + | [[Image:1qz9.jpg|left|200px]]<br /><applet load="1qz9" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1qz9, resolution 1.85Å" /> | caption="1qz9, resolution 1.85Å" /> | ||
'''The Three Dimensional Structure of Kynureninase from Pseudomonas fluorescens'''<br /> | '''The Three Dimensional Structure of Kynureninase from Pseudomonas fluorescens'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Kynureninase [E.C. 3.7.1.3] is a pyridoxal-5'-phosphate (PLP)-dependent | + | Kynureninase [E.C. 3.7.1.3] is a pyridoxal-5'-phosphate (PLP)-dependent enzyme that catalyzes the hydrolytic cleavage of l-kynurenine to anthranilic acid and l-alanine. Sequence alignment with other PLP-dependent enzymes indicated that kynureninase is in subgroup IVa of the aminotransferases, along with nifS, CsdB, and serine-pyruvate aminotransferase, which suggests that kynureninase has an aminotransferase fold. Crystals of Pseudomonas fluorescens kynureninase were obtained, and the structure was solved by molecular replacement using the CsdB coordinates combined with multiple isomorphous heavy atom replacement. The coordinates were deposited in the PDB (ID code 1QZ9). The structure, refined to an R factor of 15.5% to 1.85 A resolution, is dimeric and has the aminotransferase fold. The structure also confirms the prediction from sequence alignment that Lys-227 is the PLP-binding residue in P. fluorescens kynureninase. The conserved Asp-201, expected for an aminotransferase fold, is located near the PLP nitrogen, but Asp-132 is also strictly conserved and at a similar distance from the pyridinium nitrogen. Mutagenesis of both conserved aspartic acids shows that both contribute equally to PLP binding, but Asp-201 has a greater role in catalysis. The structure shows that Tyr-226 donates a hydrogen bond to the phosphate of PLP. Unusual among PLP-dependent enzymes, Trp-256, which is also strictly conserved in kynureninases from bacteria to humans, donates a hydrogen bond to the phosphate through the indole N1-hydrogen. |
==About this Structure== | ==About this Structure== | ||
| - | 1QZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens] with CL, PLP and P3G as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Kynureninase Kynureninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.3 3.7.1.3] Full crystallographic information is available from [http:// | + | 1QZ9 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Pseudomonas_fluorescens Pseudomonas fluorescens] with <scene name='pdbligand=CL:'>CL</scene>, <scene name='pdbligand=PLP:'>PLP</scene> and <scene name='pdbligand=P3G:'>P3G</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Kynureninase Kynureninase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.7.1.3 3.7.1.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1QZ9 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lima, S.]] | [[Category: Lima, S.]] | ||
[[Category: Momany, C.]] | [[Category: Momany, C.]] | ||
| - | [[Category: Phillips, R | + | [[Category: Phillips, R S.]] |
[[Category: CL]] | [[Category: CL]] | ||
[[Category: P3G]] | [[Category: P3G]] | ||
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[[Category: vitamin b6]] | [[Category: vitamin b6]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:19 2008'' |
Revision as of 12:45, 21 February 2008
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The Three Dimensional Structure of Kynureninase from Pseudomonas fluorescens
Overview
Kynureninase [E.C. 3.7.1.3] is a pyridoxal-5'-phosphate (PLP)-dependent enzyme that catalyzes the hydrolytic cleavage of l-kynurenine to anthranilic acid and l-alanine. Sequence alignment with other PLP-dependent enzymes indicated that kynureninase is in subgroup IVa of the aminotransferases, along with nifS, CsdB, and serine-pyruvate aminotransferase, which suggests that kynureninase has an aminotransferase fold. Crystals of Pseudomonas fluorescens kynureninase were obtained, and the structure was solved by molecular replacement using the CsdB coordinates combined with multiple isomorphous heavy atom replacement. The coordinates were deposited in the PDB (ID code 1QZ9). The structure, refined to an R factor of 15.5% to 1.85 A resolution, is dimeric and has the aminotransferase fold. The structure also confirms the prediction from sequence alignment that Lys-227 is the PLP-binding residue in P. fluorescens kynureninase. The conserved Asp-201, expected for an aminotransferase fold, is located near the PLP nitrogen, but Asp-132 is also strictly conserved and at a similar distance from the pyridinium nitrogen. Mutagenesis of both conserved aspartic acids shows that both contribute equally to PLP binding, but Asp-201 has a greater role in catalysis. The structure shows that Tyr-226 donates a hydrogen bond to the phosphate of PLP. Unusual among PLP-dependent enzymes, Trp-256, which is also strictly conserved in kynureninases from bacteria to humans, donates a hydrogen bond to the phosphate through the indole N1-hydrogen.
About this Structure
1QZ9 is a Single protein structure of sequence from Pseudomonas fluorescens with , and as ligands. Active as Kynureninase, with EC number 3.7.1.3 Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of kynureninase from Pseudomonas fluorescens., Momany C, Levdikov V, Blagova L, Lima S, Phillips RS, Biochemistry. 2004 Feb 10;43(5):1193-203. PMID:14756555
Page seeded by OCA on Thu Feb 21 14:45:19 2008
Categories: Kynureninase | Pseudomonas fluorescens | Single protein | Blagova, L. | Levdikov, V. | Lima, S. | Momany, C. | Phillips, R S. | CL | P3G | PLP | Kynurenine | Plp | Pyridoxal-5'-phosphate | Tryptophan | Vitamin b6
