1r19
From Proteopedia
(New page: 200px<br /><applet load="1r19" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r19, resolution 3.51Å" /> '''Crystal Structure An...) |
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- | [[Image:1r19.jpg|left|200px]]<br /><applet load="1r19" size=" | + | [[Image:1r19.jpg|left|200px]]<br /><applet load="1r19" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1r19, resolution 3.51Å" /> | caption="1r19, resolution 3.51Å" /> | ||
'''Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)'''<br /> | '''Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)'''<br /> | ||
==Overview== | ==Overview== | ||
- | Gram-positive pathogens such as staphylococci contain multiple cell | + | Gram-positive pathogens such as staphylococci contain multiple cell wall-anchored proteins that serve as an interface between the microbe and its environment. Some of these proteins act as adhesins and mediate bacterial attachment to host tissues. SdrG is a cell wall-anchored adhesin from Staphylococcus epidermidis that binds to the Bbeta chain of human fibrinogen (Fg) and is necessary and sufficient for bacterial attachment to Fg-coated biomaterials. Here, we present the crystal structures of the ligand binding region of SdrG as an apoprotein and in complex with a synthetic peptide analogous to its binding site in Fg. Analysis of the crystal structures, along with mutational studies of both the protein and of the peptide, reveals that SdrG binds to its ligand with a dynamic "dock, lock, and latch" mechanism. We propose that this mechanism represents a general mode of ligand binding for structurally related cell wall-anchored proteins of gram-positive bacteria. |
==About this Structure== | ==About this Structure== | ||
- | 1R19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http:// | + | 1R19 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_epidermidis Staphylococcus epidermidis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R19 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Staphylococcus epidermidis]] | [[Category: Staphylococcus epidermidis]] | ||
- | [[Category: Bowden, M | + | [[Category: Bowden, M G.]] |
[[Category: Choe, D.]] | [[Category: Choe, D.]] | ||
[[Category: Davis, S.]] | [[Category: Davis, S.]] | ||
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[[Category: Hook, M.]] | [[Category: Hook, M.]] | ||
[[Category: Moore, D.]] | [[Category: Moore, D.]] | ||
- | [[Category: Narayana, S | + | [[Category: Narayana, S V.L.]] |
[[Category: Ponnuraj, K.]] | [[Category: Ponnuraj, K.]] | ||
[[Category: Xu, Y.]] | [[Category: Xu, Y.]] | ||
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[[Category: sdrg native]] | [[Category: sdrg native]] | ||
- | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:45:56 2008'' |
Revision as of 12:45, 21 February 2008
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Crystal Structure Analysis of S.epidermidis adhesin SdrG binding to Fibrinogen (Apo structure)
Overview
Gram-positive pathogens such as staphylococci contain multiple cell wall-anchored proteins that serve as an interface between the microbe and its environment. Some of these proteins act as adhesins and mediate bacterial attachment to host tissues. SdrG is a cell wall-anchored adhesin from Staphylococcus epidermidis that binds to the Bbeta chain of human fibrinogen (Fg) and is necessary and sufficient for bacterial attachment to Fg-coated biomaterials. Here, we present the crystal structures of the ligand binding region of SdrG as an apoprotein and in complex with a synthetic peptide analogous to its binding site in Fg. Analysis of the crystal structures, along with mutational studies of both the protein and of the peptide, reveals that SdrG binds to its ligand with a dynamic "dock, lock, and latch" mechanism. We propose that this mechanism represents a general mode of ligand binding for structurally related cell wall-anchored proteins of gram-positive bacteria.
About this Structure
1R19 is a Single protein structure of sequence from Staphylococcus epidermidis. Full crystallographic information is available from OCA.
Reference
A "dock, lock, and latch" structural model for a staphylococcal adhesin binding to fibrinogen., Ponnuraj K, Bowden MG, Davis S, Gurusiddappa S, Moore D, Choe D, Xu Y, Hook M, Narayana SV, Cell. 2003 Oct 17;115(2):217-28. PMID:14567919
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