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1r3g
From Proteopedia
(New page: 200px<br /><applet load="1r3g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r3g, resolution 1.16Å" /> '''1.16A X-ray structur...) |
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| - | [[Image:1r3g.gif|left|200px]]<br /><applet load="1r3g" size=" | + | [[Image:1r3g.gif|left|200px]]<br /><applet load="1r3g" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1r3g, resolution 1.16Å" /> | caption="1r3g, resolution 1.16Å" /> | ||
'''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues'''<br /> | '''1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues'''<br /> | ||
==Overview== | ==Overview== | ||
| - | [structure: see text] Oligonucleotides with a novel | + | [structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution. |
==About this Structure== | ==About this Structure== | ||
| - | 1R3G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1R3G is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=MG:'>MG</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R3G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Lesnik, E.]] | [[Category: Lesnik, E.]] | ||
[[Category: Manoharan, M.]] | [[Category: Manoharan, M.]] | ||
| - | [[Category: Prakash, T | + | [[Category: Prakash, T P.]] |
[[Category: Puschl, A.]] | [[Category: Puschl, A.]] | ||
[[Category: Tereshko, V.]] | [[Category: Tereshko, V.]] | ||
| Line 21: | Line 21: | ||
[[Category: dna]] | [[Category: dna]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:35 2008'' |
Revision as of 12:46, 21 February 2008
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1.16A X-ray structure of the synthetic DNA fragment with the incorporated 2'-O-[(2-Guanidinium)ethyl]-5-methyluridine residues
Overview
[structure: see text] Oligonucleotides with a novel 2'-O-[2-(guanidinium)ethyl] (2'-O-GE) modification have been synthesized using a novel protecting group strategy for the guanidinium group. This modification enhances the binding affinity of oligonucleotides to RNA as well as duplex DNA (DeltaT(m) 3.2 degrees C per modification). The 2'-O-GE modified oligonucleotides exhibited exceptional resistance to nuclease degradation. The crystal structure of a palindromic duplex formed by a DNA oligonucleotide with a single 2'-O-GE modification was solved at 1.16 A resolution.
About this Structure
1R3G is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
2'-O-[2-(guanidinium)ethyl]-modified oligonucleotides: stabilizing effect on duplex and triplex structures., Prakash TP, Puschl A, Lesnik E, Mohan V, Tereshko V, Egli M, Manoharan M, Org Lett. 2004 Jun 10;6(12):1971-4. PMID:15176796
Page seeded by OCA on Thu Feb 21 14:46:35 2008
Categories: Protein complex | Egli, M. | Lesnik, E. | Manoharan, M. | Prakash, T P. | Puschl, A. | Tereshko, V. | MG | Dna
