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1r44

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(New page: 200px<br /><applet load="1r44" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r44, resolution 2.25&Aring;" /> '''Crystal Structure of...)
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[[Image:1r44.jpg|left|200px]]<br /><applet load="1r44" size="450" color="white" frame="true" align="right" spinBox="true"
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[[Image:1r44.jpg|left|200px]]<br /><applet load="1r44" size="350" color="white" frame="true" align="right" spinBox="true"
caption="1r44, resolution 2.25&Aring;" />
caption="1r44, resolution 2.25&Aring;" />
'''Crystal Structure of VanX'''<br />
'''Crystal Structure of VanX'''<br />
==Overview==
==Overview==
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VanX is a zinc-dependent D-alanyl-D-alanine dipeptidase that is a critical, component in a system that mediates transposon-based vancomycin resistance, in enterococci. It is also a key drug target in circumventing clinical, vancomycin resistance. The structure of VanX from E. faecium has been, solved by X-ray crystallography and reveals a Zn(2+)-dipeptidase with a, unique overall fold and a well-defined active site confined within a, cavity of limited size. The crystal structures of VanX, the, VanX:D-alanyl-D-alanine complex, the VanX:D-alanine complex, and VanX in, complex with phosphonate and phosphinate transition-state analog, inhibitors, are also presented at high resolution. Structural homology, searches of known structures revealed that the fold of VanX is similar to, those of two proteins: the N-terminal fragment of murine Sonic hedgehog, and the Zn(2+)-dependent N-acyl-D-alanyl-D-alanine carboxypeptidase of S., albus G.
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VanX is a zinc-dependent D-alanyl-D-alanine dipeptidase that is a critical component in a system that mediates transposon-based vancomycin resistance in enterococci. It is also a key drug target in circumventing clinical vancomycin resistance. The structure of VanX from E. faecium has been solved by X-ray crystallography and reveals a Zn(2+)-dipeptidase with a unique overall fold and a well-defined active site confined within a cavity of limited size. The crystal structures of VanX, the VanX:D-alanyl-D-alanine complex, the VanX:D-alanine complex, and VanX in complex with phosphonate and phosphinate transition-state analog inhibitors, are also presented at high resolution. Structural homology searches of known structures revealed that the fold of VanX is similar to those of two proteins: the N-terminal fragment of murine Sonic hedgehog and the Zn(2+)-dependent N-acyl-D-alanyl-D-alanine carboxypeptidase of S. albus G.
==About this Structure==
==About this Structure==
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1R44 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium] with ZN as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1R44 OCA].
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1R44 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Enterococcus_faecium Enterococcus faecium] with <scene name='pdbligand=ZN:'>ZN</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R44 OCA].
==Reference==
==Reference==
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[[Category: Holzman, T.]]
[[Category: Holzman, T.]]
[[Category: Katz, L.]]
[[Category: Katz, L.]]
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[[Category: Park, C.H.]]
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[[Category: Park, C H.]]
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[[Category: Pratt, S.D.]]
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[[Category: Pratt, S D.]]
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[[Category: Severin, J.M.]]
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[[Category: Severin, J M.]]
[[Category: ZN]]
[[Category: ZN]]
[[Category: dipeptidase]]
[[Category: dipeptidase]]
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[[Category: e.faecium]]
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[[Category: e faecium]]
[[Category: vanx]]
[[Category: vanx]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:14:54 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:46:54 2008''

Revision as of 12:46, 21 February 2008

Image:1r44.jpg

1r44, resolution 2.25Å

Drag the structure with the mouse to rotate

Crystal Structure of VanX

Overview

VanX is a zinc-dependent D-alanyl-D-alanine dipeptidase that is a critical component in a system that mediates transposon-based vancomycin resistance in enterococci. It is also a key drug target in circumventing clinical vancomycin resistance. The structure of VanX from E. faecium has been solved by X-ray crystallography and reveals a Zn(2+)-dipeptidase with a unique overall fold and a well-defined active site confined within a cavity of limited size. The crystal structures of VanX, the VanX:D-alanyl-D-alanine complex, the VanX:D-alanine complex, and VanX in complex with phosphonate and phosphinate transition-state analog inhibitors, are also presented at high resolution. Structural homology searches of known structures revealed that the fold of VanX is similar to those of two proteins: the N-terminal fragment of murine Sonic hedgehog and the Zn(2+)-dependent N-acyl-D-alanyl-D-alanine carboxypeptidase of S. albus G.

About this Structure

1R44 is a Single protein structure of sequence from Enterococcus faecium with as ligand. Full crystallographic information is available from OCA.

Reference

The structure of VanX reveals a novel amino-dipeptidase involved in mediating transposon-based vancomycin resistance., Bussiere DE, Pratt SD, Katz L, Severin JM, Holzman T, Park CH, Mol Cell. 1998 Jul;2(1):75-84. PMID:9702193

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