1r8o
From Proteopedia
(New page: 200px<br /><applet load="1r8o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r8o, resolution 1.83Å" /> '''Crystal structure of...) |
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| - | [[Image:1r8o.jpg|left|200px]]<br /><applet load="1r8o" size=" | + | [[Image:1r8o.jpg|left|200px]]<br /><applet load="1r8o" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1r8o, resolution 1.83Å" /> | caption="1r8o, resolution 1.83Å" /> | ||
'''Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds'''<br /> | '''Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystallographic structure of a novel trypsin inhibitor (CTI) from | + | The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed. |
==About this Structure== | ==About this Structure== | ||
| - | 1R8O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Copaifera_langsdorffii Copaifera langsdorffii]. Full crystallographic information is available from [http:// | + | 1R8O is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Copaifera_langsdorffii Copaifera langsdorffii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R8O OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Krauchenco, S.]] | [[Category: Krauchenco, S.]] | ||
[[Category: Marangoni, S.]] | [[Category: Marangoni, S.]] | ||
| - | [[Category: Nagem, R | + | [[Category: Nagem, R A.P.]] |
[[Category: Polikarpov, I.]] | [[Category: Polikarpov, I.]] | ||
| - | [[Category: Silva, J | + | [[Category: Silva, J A.da.]] |
[[Category: beta-trefoil fold]] | [[Category: beta-trefoil fold]] | ||
[[Category: copaifera langsdorffii]] | [[Category: copaifera langsdorffii]] | ||
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[[Category: x-ray structure]] | [[Category: x-ray structure]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:18 2008'' |
Revision as of 12:48, 21 February 2008
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Crystal structure of an unusual Kunitz-type trypsin inhibitor from Copaifera langsdorffii seeds
Overview
The crystallographic structure of a novel trypsin inhibitor (CTI) from Copaifera langsdorffii is reported. The structure was solved by MIRAS procedure and refined to a crystallographic residual of 17.3% (R(free) = 20.3%) at 1.8 A resolution. Two isomorphous derivatives were obtained by quick cryo-soaking approach. CTI is the first structure of a member of Kunitz (STI) family formed by two noncovalently bound polypeptide chains and only one disulfide bridge. A standard Kunitz-type inhibitor has a single polypeptide chain and two disulfide bridges. Structural features granting CTI high inhibitory activity are discussed.
About this Structure
1R8O is a Protein complex structure of sequences from Copaifera langsdorffii. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of an unusual Kunitz (STI) type trypsin inhibitor from Copaifera langsdorffii., Krauchenco S, Nagem RA, da Silva JA, Marangoni S, Polikarpov I, Biochimie. 2004 Mar;86(3):167-72. PMID:15134830
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