1r9g
From Proteopedia
(New page: 200px<br /><applet load="1r9g" size="450" color="white" frame="true" align="right" spinBox="true" caption="1r9g, resolution 2.5Å" /> '''Three-dimensional Str...) |
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| - | [[Image:1r9g.jpg|left|200px]]<br /><applet load="1r9g" size=" | + | [[Image:1r9g.jpg|left|200px]]<br /><applet load="1r9g" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1r9g, resolution 2.5Å" /> | caption="1r9g, resolution 2.5Å" /> | ||
'''Three-dimensional Structure of YaaE from Bacillus subtilis'''<br /> | '''Three-dimensional Structure of YaaE from Bacillus subtilis'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of YaaE from Bacillus subtilis was determined at 2.5-A | + | The structure of YaaE from Bacillus subtilis was determined at 2.5-A resolution. YaaE is a member of the triad glutamine aminotransferase family and functions in a recently identified alternate pathway for the biosynthesis of vitamin B(6). Proposed active residues include conserved Cys-79, His-170, and Glu-172. YaaE shows similarity to HisH, a glutaminase involved in histidine biosynthesis. YaaD associates with YaaE. A homology model of this protein was constructed. YaaD is predicted to be a (beta/alpha)(8) barrel on the basis of sequence comparisons. The predicted active site includes highly conserved residues 211-216 and 233-235. Finally, a homology model of a putative YaaD-YaaE complex was prepared using the structure of HisH-F as a model. This model predicts that the ammonia molecule generated by YaaE is channeled through the center of the YaaD barrel to the putative YaaD active site. |
==About this Structure== | ==About this Structure== | ||
| - | 1R9G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http:// | + | 1R9G is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1R9G OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Bacillus subtilis]] | [[Category: Bacillus subtilis]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bauer, J | + | [[Category: Bauer, J A.]] |
| - | [[Category: Begley, T | + | [[Category: Begley, T P.]] |
| - | [[Category: Bennett, E | + | [[Category: Bennett, E M.]] |
| - | [[Category: Ealick, S | + | [[Category: Ealick, S E.]] |
[[Category: alpha/beta protein]] | [[Category: alpha/beta protein]] | ||
[[Category: cofactor biosynthesis]] | [[Category: cofactor biosynthesis]] | ||
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[[Category: triad amidotransferase]] | [[Category: triad amidotransferase]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:26 2008'' |
Revision as of 12:48, 21 February 2008
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Three-dimensional Structure of YaaE from Bacillus subtilis
Overview
The structure of YaaE from Bacillus subtilis was determined at 2.5-A resolution. YaaE is a member of the triad glutamine aminotransferase family and functions in a recently identified alternate pathway for the biosynthesis of vitamin B(6). Proposed active residues include conserved Cys-79, His-170, and Glu-172. YaaE shows similarity to HisH, a glutaminase involved in histidine biosynthesis. YaaD associates with YaaE. A homology model of this protein was constructed. YaaD is predicted to be a (beta/alpha)(8) barrel on the basis of sequence comparisons. The predicted active site includes highly conserved residues 211-216 and 233-235. Finally, a homology model of a putative YaaD-YaaE complex was prepared using the structure of HisH-F as a model. This model predicts that the ammonia molecule generated by YaaE is channeled through the center of the YaaD barrel to the putative YaaD active site.
About this Structure
1R9G is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.
Reference
Three-dimensional structure of YaaE from Bacillus subtilis, a glutaminase implicated in pyridoxal-5'-phosphate biosynthesis., Bauer JA, Bennett EM, Begley TP, Ealick SE, J Biol Chem. 2004 Jan 23;279(4):2704-11. Epub 2003 Oct 28. PMID:14585832
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