1ra4
From Proteopedia
(New page: 200px<br /><applet load="1ra4" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ra4, resolution 1.86Å" /> '''Crystal structure of...) |
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| - | [[Image:1ra4.gif|left|200px]]<br /><applet load="1ra4" size=" | + | [[Image:1ra4.gif|left|200px]]<br /><applet load="1ra4" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1ra4, resolution 1.86Å" /> | caption="1ra4, resolution 1.86Å" /> | ||
'''Crystal structure of the Methanococcus jannaschii L7Ae protein'''<br /> | '''Crystal structure of the Methanococcus jannaschii L7Ae protein'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein | + | Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions. |
==About this Structure== | ==About this Structure== | ||
| - | 1RA4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http:// | + | 1RA4 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RA4 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Methanocaldococcus jannaschii]] | [[Category: Methanocaldococcus jannaschii]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Brown, B | + | [[Category: Brown, B A.]] |
| - | [[Category: Maxwell, E | + | [[Category: Maxwell, E S.]] |
[[Category: Suryadi, J.]] | [[Category: Suryadi, J.]] | ||
| - | [[Category: Tran, E | + | [[Category: Tran, E J.]] |
[[Category: alpha-beta-alpha sandwich fold]] | [[Category: alpha-beta-alpha sandwich fold]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:48:37 2008'' |
Revision as of 12:48, 21 February 2008
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Crystal structure of the Methanococcus jannaschii L7Ae protein
Overview
Archaeal ribosomal protein L7Ae is a multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal, box H/ACA, and box C/D sRNAs. The crystal structure of Methanocaldococcus jannaschii L7Ae has been determined to 1.45 A, and L7Ae's amino acid composition, evolutionary conservation, functional characteristics, and structural details have been analyzed. Comparison of the L7Ae structure to those of a number of related proteins with diverse functions has revealed significant structural homology which suggests that this protein fold is an ancient RNA-binding motif. Notably, the free M. jannaschii L7Ae structure is essentially identical to that with RNA bound, suggesting that RNA binding occurs through an induced-fit interaction. Circular dichroism experiments show that box C/D and C'/D' RNA motifs undergo conformational changes when magnesium or the L7Ae protein is added, corroborating the induced-fit model for L7Ae-box C/D RNA interactions.
About this Structure
1RA4 is a Single protein structure of sequence from Methanocaldococcus jannaschii. Full crystallographic information is available from OCA.
Reference
The crystal structure of the Methanocaldococcus jannaschii multifunctional L7Ae RNA-binding protein reveals an induced-fit interaction with the box C/D RNAs., Suryadi J, Tran EJ, Maxwell ES, Brown BA 2nd, Biochemistry. 2005 Jul 19;44(28):9657-72. PMID:16008351
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