1rjv
From Proteopedia
(New page: 200px<br /> <applet load="1rjv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rjv" /> '''Solution Structure of Human alpha-Parvalbum...) |
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'''Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy'''<br /> | '''Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy'''<br /> | ||
==Overview== | ==Overview== | ||
| - | In the frame of a research aimed at the detailed structural | + | In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition. |
==About this Structure== | ==About this Structure== | ||
| - | 1RJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1RJV is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RJV OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Baig, I.]] | [[Category: Baig, I.]] | ||
[[Category: Bertini, I.]] | [[Category: Bertini, I.]] | ||
| - | [[Category: Bianco, C | + | [[Category: Bianco, C Del.]] |
| - | [[Category: Gupta, Y | + | [[Category: Gupta, Y K.]] |
| - | [[Category: Lee, Y | + | [[Category: Lee, Y M.]] |
[[Category: Luchinat, C.]] | [[Category: Luchinat, C.]] | ||
[[Category: Quattrone, A.]] | [[Category: Quattrone, A.]] | ||
| - | [[Category: SPINE, Structural | + | [[Category: SPINE, Structural Proteomics in Europe.]] |
[[Category: CA]] | [[Category: CA]] | ||
[[Category: calcium]] | [[Category: calcium]] | ||
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[[Category: structural proteomics in europe]] | [[Category: structural proteomics in europe]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:38 2008'' |
Revision as of 12:51, 21 February 2008
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Solution Structure of Human alpha-Parvalbumin refined with a paramagnetism-based strategy
Overview
In the frame of a research aimed at the detailed structural characterization of human calcium-binding proteins of the EF-hand family, the solution structure of human alpha-parvalbumin has been solved by NMR and refined with the help of substitution of the Ca(2+) ion in the EF site with the paramagnetic Dy(3+) ion. A simple (1)H-(15)N HSQC spectrum allowed the NH assignments based on the properties of Dy(3+). This allowed us to exploit pseudocontact shifts and residual dipolar couplings for solution structure refinement. The backbone and heavy atom RMSD are 0.55 +/- 0.08 and 1.02 +/- 0.08 A, respectively, and decrease to 0.39 +/- 0.05 and 0.90 +/- 0.06 A upon refinement with paramagnetism-based restraints. The RMSD for the metal itself in the EF site in the refined structure is 0.26 +/- 0.12 A. Backbone NH R(1), R(2), and NOE measured at two temperatures show the protein to be relatively rigid. The NH orientations are well determined by the paramagnetism-based restraints. This allows us to detect small but significant local structural differences with the orthologue protein from rat, whose X-ray structure is available at 2.0 A resolution. All differences are related to local changes in the amino acidic composition.
About this Structure
1RJV is a Single protein structure of sequence from Homo sapiens with as ligand. Full crystallographic information is available from OCA.
Reference
Paramagnetism-based refinement strategy for the solution structure of human alpha-parvalbumin., Baig I, Bertini I, Del Bianco C, Gupta YK, Lee YM, Luchinat C, Quattrone A, Biochemistry. 2004 May 11;43(18):5562-73. PMID:15122922
Page seeded by OCA on Thu Feb 21 14:51:38 2008
Categories: Homo sapiens | Single protein | Baig, I. | Bertini, I. | Bianco, C Del. | Gupta, Y K. | Lee, Y M. | Luchinat, C. | Quattrone, A. | SPINE, Structural Proteomics in Europe. | CA | Calcium | Ef-hand | Lanthanide | Nmr | Parvalbumin | Spine | Structural genomics | Structural proteomics in europe
