1rk8
From Proteopedia
(New page: 200px<br /><applet load="1rk8" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rk8, resolution 1.90Å" /> '''Structure of the cyt...) |
|||
| Line 1: | Line 1: | ||
| - | [[Image:1rk8.gif|left|200px]]<br /><applet load="1rk8" size=" | + | [[Image:1rk8.gif|left|200px]]<br /><applet load="1rk8" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rk8, resolution 1.90Å" /> | caption="1rk8, resolution 1.90Å" /> | ||
'''Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex'''<br /> | '''Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The exon junction complex (EJC) is deposited on mRNAs as a consequence of | + | The exon junction complex (EJC) is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. The Mago-Y14 heterodimer is a core component of the EJC. Recently, the protein PYM has been identified as an interacting partner of Mago-Y14. Here we show that PYM is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. PYM interacts directly with Mago-Y14 by means of its N-terminal domain. The crystal structure of the Drosophila ternary complex at 1.9 A resolution reveals that PYM binds Mago and Y14 simultaneously, capping their heterodimerization interface at conserved surface residues. Formation of this ternary complex is also observed with the human proteins. Mago residues involved in the interaction with PYM have been implicated in nonsense-mediated mRNA decay (NMD). Consistently, human PYM is active in NMD tethering assays. Together, these data suggest a role for PYM in NMD. |
==About this Structure== | ==About this Structure== | ||
| - | 1RK8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with CA as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1RK8 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] with <scene name='pdbligand=CA:'>CA</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RK8 OCA]. |
==Reference== | ==Reference== | ||
| Line 21: | Line 21: | ||
[[Category: mrna processing; rrm; rbd; nmd; oskar mrna localization]] | [[Category: mrna processing; rrm; rbd; nmd; oskar mrna localization]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:46 2008'' |
Revision as of 12:51, 21 February 2008
|
Structure of the cytosolic protein PYM bound to the Mago-Y14 core of the exon junction complex
Overview
The exon junction complex (EJC) is deposited on mRNAs as a consequence of splicing and influences postsplicing mRNA metabolism. The Mago-Y14 heterodimer is a core component of the EJC. Recently, the protein PYM has been identified as an interacting partner of Mago-Y14. Here we show that PYM is a cytoplasmic RNA-binding protein that is excluded from the nucleus by Crm1. PYM interacts directly with Mago-Y14 by means of its N-terminal domain. The crystal structure of the Drosophila ternary complex at 1.9 A resolution reveals that PYM binds Mago and Y14 simultaneously, capping their heterodimerization interface at conserved surface residues. Formation of this ternary complex is also observed with the human proteins. Mago residues involved in the interaction with PYM have been implicated in nonsense-mediated mRNA decay (NMD). Consistently, human PYM is active in NMD tethering assays. Together, these data suggest a role for PYM in NMD.
About this Structure
1RK8 is a Protein complex structure of sequences from Drosophila melanogaster with as ligand. Full crystallographic information is available from OCA.
Reference
Molecular insights into the interaction of PYM with the Mago-Y14 core of the exon junction complex., Bono F, Ebert J, Unterholzner L, Guttler T, Izaurralde E, Conti E, EMBO Rep. 2004 Mar;5(3):304-10. Epub 2004 Feb 13. PMID:14968132
Page seeded by OCA on Thu Feb 21 14:51:46 2008
