1rk4
From Proteopedia
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==Overview== | ==Overview== | ||
| - | Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least | + | Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least two stable conformations. Recently, a class of intracellular chloride ion channel proteins (CLICs) has been shown to exist in both soluble and integral membrane forms. The structure of the soluble form of CLIC1 is typical of a soluble glutathione S-transferase superfamily protein but contains a glutaredoxin-like active site. In this study we show that on oxidation CLIC1 undergoes a reversible transition from a monomeric to a non-covalent dimeric state due to the formation of an intramolecular disulfide bond (Cys-24-Cys-59). We have determined the crystal structure of this oxidized state and show that a major structural transition has occurred, exposing a large hydrophobic surface, which forms the dimer interface. The oxidized CLIC1 dimer maintains its ability to form chloride ion channels in artificial bilayers and vesicles, whereas a reducing environment prevents the formation of ion channels by CLIC1. Mutational studies show that both Cys-24 and Cys-59 are required for channel activity. |
==About this Structure== | ==About this Structure== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bauskin, A | + | [[Category: Bauskin, A R.]] |
| - | [[Category: Breit, S | + | [[Category: Breit, S N.]] |
| - | [[Category: Brown, L | + | [[Category: Brown, L J.]] |
| - | [[Category: Campbell, T | + | [[Category: Campbell, T J.]] |
| - | [[Category: Curmi, P | + | [[Category: Curmi, P M.]] |
| - | [[Category: DeMaere, M | + | [[Category: DeMaere, M Z.]] |
| - | [[Category: Fairlie, W | + | [[Category: Fairlie, W D.]] |
| - | [[Category: Harrop, S | + | [[Category: Harrop, S J.]] |
| - | [[Category: Littler, D | + | [[Category: Littler, D R.]] |
[[Category: Mazzanti, M.]] | [[Category: Mazzanti, M.]] | ||
| - | [[Category: Pankhurst, G | + | [[Category: Pankhurst, G J.]] |
[[Category: Pankhurst, S.]] | [[Category: Pankhurst, S.]] | ||
[[Category: Tonini, R.]] | [[Category: Tonini, R.]] | ||
[[Category: glutathione-s-tranferase superfamily; chloride ion channel; redox-controlled structural transition]] | [[Category: glutathione-s-tranferase superfamily; chloride ion channel; redox-controlled structural transition]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:51:46 2008'' |
Revision as of 12:51, 21 February 2008
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Crystal Structure of a Soluble Dimeric Form of Oxidised CLIC1
Overview
Most proteins adopt a well defined three-dimensional structure; however, it is increasingly recognized that some proteins can exist with at least two stable conformations. Recently, a class of intracellular chloride ion channel proteins (CLICs) has been shown to exist in both soluble and integral membrane forms. The structure of the soluble form of CLIC1 is typical of a soluble glutathione S-transferase superfamily protein but contains a glutaredoxin-like active site. In this study we show that on oxidation CLIC1 undergoes a reversible transition from a monomeric to a non-covalent dimeric state due to the formation of an intramolecular disulfide bond (Cys-24-Cys-59). We have determined the crystal structure of this oxidized state and show that a major structural transition has occurred, exposing a large hydrophobic surface, which forms the dimer interface. The oxidized CLIC1 dimer maintains its ability to form chloride ion channels in artificial bilayers and vesicles, whereas a reducing environment prevents the formation of ion channels by CLIC1. Mutational studies show that both Cys-24 and Cys-59 are required for channel activity.
About this Structure
1RK4 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The intracellular chloride ion channel protein CLIC1 undergoes a redox-controlled structural transition., Littler DR, Harrop SJ, Fairlie WD, Brown LJ, Pankhurst GJ, Pankhurst S, DeMaere MZ, Campbell TJ, Bauskin AR, Tonini R, Mazzanti M, Breit SN, Curmi PM, J Biol Chem. 2004 Mar 5;279(10):9298-305. Epub 2003 Nov 12. PMID:14613939
Page seeded by OCA on Thu Feb 21 14:51:46 2008
Categories: Homo sapiens | Single protein | Bauskin, A R. | Breit, S N. | Brown, L J. | Campbell, T J. | Curmi, P M. | DeMaere, M Z. | Fairlie, W D. | Harrop, S J. | Littler, D R. | Mazzanti, M. | Pankhurst, G J. | Pankhurst, S. | Tonini, R. | Glutathione-s-tranferase superfamily; chloride ion channel; redox-controlled structural transition
