1rl1
From Proteopedia
(New page: 200px<br /> <applet load="1rl1" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rl1" /> '''Solution structure of human Sgt1 CS domain'...) |
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'''Solution structure of human Sgt1 CS domain'''<br /> | '''Solution structure of human Sgt1 CS domain'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Sgt1 has been identified as a subunit of both core kinetochore and SCF | + | Sgt1 has been identified as a subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes and is also implicated in plant disease resistance. Sgt1 has two putative HSP90 binding domains, a tetratricopeptide repeat and a p23-like CHORD and Sgt1 (CS) domain. Using NMR spectroscopy, we show that only the CS domain of human Sgt1 physically interacts with HSP90. The tetratricopeptide repeat domain does not bind to either HSP90 or HSP70. Determination of the three-dimensional structure showed that the Sgt1-CS domain shares the same beta-sandwich fold as p23 but lacks the last highly conserved beta-strand in p23. Analysis of the structures of Sgt1-CS and p23 revealed a similar charge distribution on one of two opposing surfaces that suggests that it is the binding region for HSP90 in Sgt1. Although ATP is absolutely required for p23 binding to HSP90, Sgt1 binds to HSP90 also in the absence of the non-hydrolyzable analog ATPgammaS. Our findings suggest the CS domain is a binding module for HSP90 distinct from p23-like domains, which implies that Sgt1 and related proteins function in recruiting heat shock protein activities to multiprotein assemblies. |
==About this Structure== | ==About this Structure== | ||
| - | 1RL1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1RL1 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RL1 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Chazin, W | + | [[Category: Chazin, W J.]] |
[[Category: Jacob, J.]] | [[Category: Jacob, J.]] | ||
[[Category: Kuznicki, J.]] | [[Category: Kuznicki, J.]] | ||
| - | [[Category: Lee, Y | + | [[Category: Lee, Y T.]] |
[[Category: Michowski, W.]] | [[Category: Michowski, W.]] | ||
[[Category: Nowotny, M.]] | [[Category: Nowotny, M.]] | ||
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[[Category: similar to p23]] | [[Category: similar to p23]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:52:02 2008'' |
Revision as of 12:52, 21 February 2008
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Solution structure of human Sgt1 CS domain
Overview
Sgt1 has been identified as a subunit of both core kinetochore and SCF (Skp1-Cul1-F-box) ubiquitin ligase complexes and is also implicated in plant disease resistance. Sgt1 has two putative HSP90 binding domains, a tetratricopeptide repeat and a p23-like CHORD and Sgt1 (CS) domain. Using NMR spectroscopy, we show that only the CS domain of human Sgt1 physically interacts with HSP90. The tetratricopeptide repeat domain does not bind to either HSP90 or HSP70. Determination of the three-dimensional structure showed that the Sgt1-CS domain shares the same beta-sandwich fold as p23 but lacks the last highly conserved beta-strand in p23. Analysis of the structures of Sgt1-CS and p23 revealed a similar charge distribution on one of two opposing surfaces that suggests that it is the binding region for HSP90 in Sgt1. Although ATP is absolutely required for p23 binding to HSP90, Sgt1 binds to HSP90 also in the absence of the non-hydrolyzable analog ATPgammaS. Our findings suggest the CS domain is a binding module for HSP90 distinct from p23-like domains, which implies that Sgt1 and related proteins function in recruiting heat shock protein activities to multiprotein assemblies.
About this Structure
1RL1 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Human Sgt1 binds HSP90 through the CHORD-Sgt1 domain and not the tetratricopeptide repeat domain., Lee YT, Jacob J, Michowski W, Nowotny M, Kuznicki J, Chazin WJ, J Biol Chem. 2004 Apr 16;279(16):16511-7. Epub 2004 Feb 3. PMID:14761955
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