1rna
From Proteopedia
(New page: 200px<br /><applet load="1rna" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rna, resolution 2.250Å" /> '''CRYSTALLOGRAPHIC ST...) |
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| - | [[Image:1rna.gif|left|200px]]<br /><applet load="1rna" size=" | + | [[Image:1rna.gif|left|200px]]<br /><applet load="1rna" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rna, resolution 2.250Å" /> | caption="1rna, resolution 2.250Å" /> | ||
'''CRYSTALLOGRAPHIC STRUCTURE OF AN RNA HELIX: [U(U-A)6A]2'''<br /> | '''CRYSTALLOGRAPHIC STRUCTURE OF AN RNA HELIX: [U(U-A)6A]2'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The crystallographic structure of the synthetic oligoribonucleotide, U(UA)6A, has been solved at 2.25 A resolution. The crystallographic | + | The crystallographic structure of the synthetic oligoribonucleotide, U(UA)6A, has been solved at 2.25 A resolution. The crystallographic refinement permitted the identification of 91 solvent molecules, with a final agreement factor of 13%. The molecule is a dimer of 14 base-pairs and shows the typical features of an A-type helix. However, the presence of two kinks causes a divergence from a straight helix. The observed deformation, which is stabilized by a few hydrogen bonds in the crystal packing, could be due to the relatively high (35 degrees C) temperature of crystallization. The complete analysis of the structure is presented. It includes the stacking geometries, the backbone conformation and the solvation. |
==About this Structure== | ==About this Structure== | ||
| - | 1RNA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http:// | + | 1RNA is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RNA OCA]. |
==Reference== | ==Reference== | ||
Crystallographic structure of an RNA helix: [U(UA)6A]2., Dock-Bregeon AC, Chevrier B, Podjarny A, Johnson J, de Bear JS, Gough GR, Gilham PT, Moras D, J Mol Biol. 1989 Oct 5;209(3):459-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2479753 2479753] | Crystallographic structure of an RNA helix: [U(UA)6A]2., Dock-Bregeon AC, Chevrier B, Podjarny A, Johnson J, de Bear JS, Gough GR, Gilham PT, Moras D, J Mol Biol. 1989 Oct 5;209(3):459-74. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2479753 2479753] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Bear, J | + | [[Category: Bear, J S.De.]] |
[[Category: Chevrier, B.]] | [[Category: Chevrier, B.]] | ||
| - | [[Category: Dock-Bregeon, A | + | [[Category: Dock-Bregeon, A C.]] |
| - | [[Category: Gilham, P | + | [[Category: Gilham, P T.]] |
| - | [[Category: Gough, G | + | [[Category: Gough, G R.]] |
[[Category: Johnson, J.]] | [[Category: Johnson, J.]] | ||
[[Category: Moras, D.]] | [[Category: Moras, D.]] | ||
| Line 23: | Line 23: | ||
[[Category: double helix]] | [[Category: double helix]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:52:37 2008'' |
Revision as of 12:52, 21 February 2008
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CRYSTALLOGRAPHIC STRUCTURE OF AN RNA HELIX: [U(U-A)6A]2
Overview
The crystallographic structure of the synthetic oligoribonucleotide, U(UA)6A, has been solved at 2.25 A resolution. The crystallographic refinement permitted the identification of 91 solvent molecules, with a final agreement factor of 13%. The molecule is a dimer of 14 base-pairs and shows the typical features of an A-type helix. However, the presence of two kinks causes a divergence from a straight helix. The observed deformation, which is stabilized by a few hydrogen bonds in the crystal packing, could be due to the relatively high (35 degrees C) temperature of crystallization. The complete analysis of the structure is presented. It includes the stacking geometries, the backbone conformation and the solvation.
About this Structure
1RNA is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Crystallographic structure of an RNA helix: [U(UA)6A]2., Dock-Bregeon AC, Chevrier B, Podjarny A, Johnson J, de Bear JS, Gough GR, Gilham PT, Moras D, J Mol Biol. 1989 Oct 5;209(3):459-74. PMID:2479753
Page seeded by OCA on Thu Feb 21 14:52:37 2008
