1rru
From Proteopedia
(New page: 200px<br /><applet load="1rru" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rru, resolution 2.35Å" /> '''The influence of a c...) |
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| - | [[Image:1rru.gif|left|200px]]<br /><applet load="1rru" size=" | + | [[Image:1rru.gif|left|200px]]<br /><applet load="1rru" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rru, resolution 2.35Å" /> | caption="1rru, resolution 2.35Å" /> | ||
'''The influence of a chiral amino acid on the helical handedness of PNA in solution and in crystals'''<br /> | '''The influence of a chiral amino acid on the helical handedness of PNA in solution and in crystals'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The X-ray structure of a self-complementary PNA hexamer | + | The X-ray structure of a self-complementary PNA hexamer (H-CGTACG-L-Lys-NH(2)) has been determined to 2.35 A resolution. The introduction of an L-lysine moiety has previously been shown to induce a preferred left-handedness of the PNA double helices in aqueous solution. However, in the crystal structure an equal amount of interchanging right- and left-handed helices is observed. The lysine moieties are pointing into large solvent channels and no significant interactions between this moiety and the remaining PNA molecule are observed. In contrast, molecular mechanics calculations show a preference for the left-handed helix of this hexameric PNA in aqueous solution as expected. The calculations indicate that the difference in the free energy of solvation between the left-handed and the right-handed helix is the determining factor for the preference of the left-handed helix in aqueous solution. |
==About this Structure== | ==About this Structure== | ||
| - | 1RRU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with NH2 as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http:// | + | 1RRU is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with <scene name='pdbligand=NH2:'>NH2</scene> as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RRU OCA]. |
==Reference== | ==Reference== | ||
The influence of a chiral amino acid on the helical handedness of PNA in solution and in crystals., Rasmussen H, Liljefors T, Petersson B, Nielsen PE, Liljefors T, Kastrup JS, J Biomol Struct Dyn. 2004 Feb;21(4):495-502. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14692794 14692794] | The influence of a chiral amino acid on the helical handedness of PNA in solution and in crystals., Rasmussen H, Liljefors T, Petersson B, Nielsen PE, Liljefors T, Kastrup JS, J Biomol Struct Dyn. 2004 Feb;21(4):495-502. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=14692794 14692794] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Kastrup, J | + | [[Category: Kastrup, J S.]] |
[[Category: Liljefors, T.]] | [[Category: Liljefors, T.]] | ||
| - | [[Category: Nielsen, P | + | [[Category: Nielsen, P E.]] |
[[Category: Petersson, B.]] | [[Category: Petersson, B.]] | ||
[[Category: Rasmussen, H.]] | [[Category: Rasmussen, H.]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:53:55 2008'' |
Revision as of 12:53, 21 February 2008
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The influence of a chiral amino acid on the helical handedness of PNA in solution and in crystals
Overview
The X-ray structure of a self-complementary PNA hexamer (H-CGTACG-L-Lys-NH(2)) has been determined to 2.35 A resolution. The introduction of an L-lysine moiety has previously been shown to induce a preferred left-handedness of the PNA double helices in aqueous solution. However, in the crystal structure an equal amount of interchanging right- and left-handed helices is observed. The lysine moieties are pointing into large solvent channels and no significant interactions between this moiety and the remaining PNA molecule are observed. In contrast, molecular mechanics calculations show a preference for the left-handed helix of this hexameric PNA in aqueous solution as expected. The calculations indicate that the difference in the free energy of solvation between the left-handed and the right-handed helix is the determining factor for the preference of the left-handed helix in aqueous solution.
About this Structure
1RRU is a Protein complex structure of sequences from [1] with as ligand. Full crystallographic information is available from OCA.
Reference
The influence of a chiral amino acid on the helical handedness of PNA in solution and in crystals., Rasmussen H, Liljefors T, Petersson B, Nielsen PE, Liljefors T, Kastrup JS, J Biomol Struct Dyn. 2004 Feb;21(4):495-502. PMID:14692794
Page seeded by OCA on Thu Feb 21 14:53:55 2008
