1rsn
From Proteopedia
(New page: 200px<br /><applet load="1rsn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rsn, resolution 2.0Å" /> '''RIBONUCLEASE (RNASE S...) |
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| - | [[Image:1rsn.gif|left|200px]]<br /><applet load="1rsn" size=" | + | [[Image:1rsn.gif|left|200px]]<br /><applet load="1rsn" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rsn, resolution 2.0Å" /> | caption="1rsn, resolution 2.0Å" /> | ||
'''RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE'''<br /> | '''RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The structure of the complex of ribonuclease from Streptomyces | + | The structure of the complex of ribonuclease from Streptomyces aureofaciens (RNase Sa) with exo guanosine 2',3'-cyclophosphorothioate has been refined against 0.2-nm resolution synchrotron data using, as a starting model, coordinates from the RNase Sa: 2'-GMP complex. The refinement was based on all data over 1.0-0.2 nm and converged to a crystallographic R factor of 11.9%. This is the first structure of a microbial ribonuclease complexed with a 2',3'-cyclophosphorothioate, which is a thio analogue of the intermediate of the two-step reaction. However, exo guanosine 2',3'-cyclophosphorothioate is bound in a non-functional mode and is not hydrolysed. This structure therefore does not provide direct evidence on the identity of the amino acid residues responsible for catalytic cleavage of the substrate. However, based on present and previous results, a plausible model is proposed for the complex of the cyclic intermediate which acts as substrate for the second step of the catalysis. |
==About this Structure== | ==About this Structure== | ||
| - | 1RSN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens] with SO4 and SGP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Full crystallographic information is available from [http:// | + | 1RSN is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_aureofaciens Streptomyces aureofaciens] with <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=SGP:'>SGP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Ribonuclease_T(1) Ribonuclease T(1)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.3 3.1.27.3] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RSN OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Streptomyces aureofaciens]] | [[Category: Streptomyces aureofaciens]] | ||
[[Category: Dauter, Z.]] | [[Category: Dauter, Z.]] | ||
| - | [[Category: Lamzin, V | + | [[Category: Lamzin, V S.]] |
[[Category: Sevcik, J.]] | [[Category: Sevcik, J.]] | ||
| - | [[Category: Wilson, K | + | [[Category: Wilson, K S.]] |
[[Category: SGP]] | [[Category: SGP]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
[[Category: hydrolase (guanyloribonuclease)]] | [[Category: hydrolase (guanyloribonuclease)]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:54:04 2008'' |
Revision as of 12:54, 21 February 2008
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RIBONUCLEASE (RNASE SA) (E.C.3.1.4.8) COMPLEXED WITH EXO-2',3'-CYCLOPHOSPHOROTHIOATE
Overview
The structure of the complex of ribonuclease from Streptomyces aureofaciens (RNase Sa) with exo guanosine 2',3'-cyclophosphorothioate has been refined against 0.2-nm resolution synchrotron data using, as a starting model, coordinates from the RNase Sa: 2'-GMP complex. The refinement was based on all data over 1.0-0.2 nm and converged to a crystallographic R factor of 11.9%. This is the first structure of a microbial ribonuclease complexed with a 2',3'-cyclophosphorothioate, which is a thio analogue of the intermediate of the two-step reaction. However, exo guanosine 2',3'-cyclophosphorothioate is bound in a non-functional mode and is not hydrolysed. This structure therefore does not provide direct evidence on the identity of the amino acid residues responsible for catalytic cleavage of the substrate. However, based on present and previous results, a plausible model is proposed for the complex of the cyclic intermediate which acts as substrate for the second step of the catalysis.
About this Structure
1RSN is a Single protein structure of sequence from Streptomyces aureofaciens with and as ligands. Active as Ribonuclease T(1), with EC number 3.1.27.3 Full crystallographic information is available from OCA.
Reference
Complex of ribonuclease Sa with a cyclic nucleotide and a proposed model for the reaction intermediate., Sevcik J, Zegers I, Wyns L, Dauter Z, Wilson KS, Eur J Biochem. 1993 Aug 15;216(1):301-5. PMID:8396032
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