1rtq
From Proteopedia
(New page: 200px<br /><applet load="1rtq" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rtq, resolution 0.95Å" /> '''The 0.95 Angstrom Re...) |
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| - | [[Image:1rtq.gif|left|200px]]<br /><applet load="1rtq" size=" | + | [[Image:1rtq.gif|left|200px]]<br /><applet load="1rtq" size="350" color="white" frame="true" align="right" spinBox="true" |
caption="1rtq, resolution 0.95Å" /> | caption="1rtq, resolution 0.95Å" /> | ||
'''The 0.95 Angstrom Resolution Crystal Structure of the Aminopeptidase from Aeromonas proteolytica'''<br /> | '''The 0.95 Angstrom Resolution Crystal Structure of the Aminopeptidase from Aeromonas proteolytica'''<br /> | ||
==Overview== | ==Overview== | ||
| - | The aminopeptidase from Aeromonas proteolytica (AAP) contains two zinc | + | The aminopeptidase from Aeromonas proteolytica (AAP) contains two zinc ions in the active site and catalyzes the degradation of peptides. Herein we report the crystal structures of AAP at 0.95-A resolution at neutral pH and at 1.24-A resolution at low pH. The combination of these structures allowed the precise modeling of atomic positions, the identification of the metal bridging oxygen species, and insight into the physical properties of the metal ions. On the basis of these structures, a new putative catalytic mechanism is proposed for AAP that is likely relevant to all binuclear metalloproteases. |
==About this Structure== | ==About this Structure== | ||
| - | 1RTQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus] with SCN, ZN and NA as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Bacterial_leucyl_aminopeptidase Bacterial leucyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.10 3.4.11.10] Full crystallographic information is available from [http:// | + | 1RTQ is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Vibrio_proteolyticus Vibrio proteolyticus] with <scene name='pdbligand=SCN:'>SCN</scene>, <scene name='pdbligand=ZN:'>ZN</scene> and <scene name='pdbligand=NA:'>NA</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Bacterial_leucyl_aminopeptidase Bacterial leucyl aminopeptidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.11.10 3.4.11.10] Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RTQ OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Vibrio proteolyticus]] | [[Category: Vibrio proteolyticus]] | ||
| - | [[Category: Bienvenue, D | + | [[Category: Bienvenue, D L.]] |
[[Category: Desmarais, W.]] | [[Category: Desmarais, W.]] | ||
| - | [[Category: Holz, R | + | [[Category: Holz, R C.]] |
| - | [[Category: Krzysztof, B | + | [[Category: Krzysztof, B P.]] |
| - | [[Category: Petsko, G | + | [[Category: Petsko, G A.]] |
[[Category: Ringe, D.]] | [[Category: Ringe, D.]] | ||
[[Category: NA]] | [[Category: NA]] | ||
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[[Category: zinc]] | [[Category: zinc]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:54:27 2008'' |
Revision as of 12:54, 21 February 2008
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The 0.95 Angstrom Resolution Crystal Structure of the Aminopeptidase from Aeromonas proteolytica
Overview
The aminopeptidase from Aeromonas proteolytica (AAP) contains two zinc ions in the active site and catalyzes the degradation of peptides. Herein we report the crystal structures of AAP at 0.95-A resolution at neutral pH and at 1.24-A resolution at low pH. The combination of these structures allowed the precise modeling of atomic positions, the identification of the metal bridging oxygen species, and insight into the physical properties of the metal ions. On the basis of these structures, a new putative catalytic mechanism is proposed for AAP that is likely relevant to all binuclear metalloproteases.
About this Structure
1RTQ is a Single protein structure of sequence from Vibrio proteolyticus with , and as ligands. Active as Bacterial leucyl aminopeptidase, with EC number 3.4.11.10 Full crystallographic information is available from OCA.
Reference
The high-resolution structures of the neutral and the low pH crystals of aminopeptidase from Aeromonas proteolytica., Desmarais W, Bienvenue DL, Bzymek KP, Petsko GA, Ringe D, Holz RC, J Biol Inorg Chem. 2006 Jun;11(4):398-408. Epub 2006 Apr 5. PMID:16596389
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