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1rw3

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(New page: 200px<br /><applet load="1rw3" size="450" color="white" frame="true" align="right" spinBox="true" caption="1rw3, resolution 3.0&Aring;" /> '''The crystal structure...)
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'''The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus'''<br />
'''The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus'''<br />
==Overview==
==Overview==
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Reverse transcriptases (RTs) are multidomain enzymes of variable, architecture that couple both RNA- and DNA-directed DNA polymerase, activities with an RNase H activity specific for an RNA:DNA hybrid in, order to replicate the single-stranded RNA genome of the retrovirus., Previous structural work has been reported for the heterodimeric HIV-1 and, HIV-2 RTs. We now report the first crystal structure of the full-length, Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure, reveals a clamp-shaped molecule resulting from the relative positions of, the thumb, connection, and RNase H domains that is strikingly different, from the HIV-1 RT and provides the first example of a monomeric reverse, transcriptase. A comparative analysis with related DNA polymerases, suggests a unique trajectory for the template-primer exiting the, polymerase active site and provides insights regarding processive DNA, synthesis by MMLV RT.
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Reverse transcriptases (RTs) are multidomain enzymes of variable architecture that couple both RNA- and DNA-directed DNA polymerase activities with an RNase H activity specific for an RNA:DNA hybrid in order to replicate the single-stranded RNA genome of the retrovirus. Previous structural work has been reported for the heterodimeric HIV-1 and HIV-2 RTs. We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. A comparative analysis with related DNA polymerases suggests a unique trajectory for the template-primer exiting the polymerase active site and provides insights regarding processive DNA synthesis by MMLV RT.
==About this Structure==
==About this Structure==
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1RW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].
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1RW3 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Moloney_murine_leukemia_virus Moloney murine leukemia virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1RW3 OCA].
==Reference==
==Reference==
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[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Das, D.]]
[[Category: Das, D.]]
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[[Category: Georgiadis, M.M.]]
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[[Category: Georgiadis, M M.]]
[[Category: reverse transcriptase]]
[[Category: reverse transcriptase]]
[[Category: rna and dna dependent dna polymerase]]
[[Category: rna and dna dependent dna polymerase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Wed Nov 21 01:54:02 2007''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 14:55:08 2008''

Revision as of 12:55, 21 February 2008

Image:1rw3.gif

1rw3, resolution 3.0Å

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The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus

Overview

Reverse transcriptases (RTs) are multidomain enzymes of variable architecture that couple both RNA- and DNA-directed DNA polymerase activities with an RNase H activity specific for an RNA:DNA hybrid in order to replicate the single-stranded RNA genome of the retrovirus. Previous structural work has been reported for the heterodimeric HIV-1 and HIV-2 RTs. We now report the first crystal structure of the full-length Moloney murine leukemia virus (MMLV) RT at 3.0 A resolution. The structure reveals a clamp-shaped molecule resulting from the relative positions of the thumb, connection, and RNase H domains that is strikingly different from the HIV-1 RT and provides the first example of a monomeric reverse transcriptase. A comparative analysis with related DNA polymerases suggests a unique trajectory for the template-primer exiting the polymerase active site and provides insights regarding processive DNA synthesis by MMLV RT.

About this Structure

1RW3 is a Single protein structure of sequence from Moloney murine leukemia virus. Full crystallographic information is available from OCA.

Reference

The crystal structure of the monomeric reverse transcriptase from Moloney murine leukemia virus., Das D, Georgiadis MM, Structure. 2004 May;12(5):819-29. PMID:15130474

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